Continuous Lactate Measurement that Combines a Portable Ultrafiltration Storage Device with an Enzyme Sensor

Cheng, Shong; Kaptein, Wilhelmina A.; Gruendig, Bernd; Yuen, Yvonne; Korf, Jakob; Renneberg, Reinhard

doi:10.1080/00032710008543180

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…We used biosensor technology for assays. Recently, nanoliter-sized sensors have been described that are compatible with the here described collection device. − The device mayalso be useful for monitoring of other low molecular weight hydrophilic analytes, such as electrolytes, drugs, or their metabolites. Possible compatible analytic techniques are flow-injection analysis, flame-photometry, GC/MS, capillary zone electrophoresis, and fluorescence. ,− Finally, the device may also be applied to collect (sterile) samples in vitro for example, from cell cultures and industrial bioreactors.…”

Section: Discussionmentioning

confidence: 99%

Minimally Invasive Technique Based on Ultraslow Ultrafiltration To Collect and Store Time Profiles of Analytes

Savenije¹,

Venema²,

Gerritzen³

et al. 2003

Anal. Chem.

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A device is described to collect and store continuously time profiles of analytes over periods of 24 h suitable to sample freely moving individuals (humans and animals). The device consists of a hollow fiber ultrafiltration probe, a long capillary and a nonmechanical unit (a disposable medical syringe) driven by vacuum to withdraw fluid. The principle is that at low rates (< or = 100 nL/min), sample fluid is collected through the ultrafiltration probe into the capillary. A time resolution of less than 5 min over a 24-h collection and storage period was achieved for lactate and glucose. To illustrate an in vivo application, devices were fixed under the wing of freely moving broiler chickens, with subcutaneous or intravenous probe placements. The device can be produced as a disposable, and it may become applied for ex vivo and in vitro monitoring.

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Section: Discussionmentioning

confidence: 99%

Minimally Invasive Technique Based on Ultraslow Ultrafiltration To Collect and Store Time Profiles of Analytes

Savenije¹,

Venema²,

Gerritzen³

et al. 2003

Anal. Chem.

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“…To stabilize the ultrafiltration flow, a defined capillary restriction (l＝4 cm, o.d.＝0.2 mm, i.d.＝0.03 mm fused silica tube) was placed in front of the syringe. 31,32 The ultrafiltration flow rate was controlled by modifying both the volume of the syringe and the length of the restriction. The ultrafiltration flow rate was on an average 5.0 µL•min -1 .…”

Section: Apparatusmentioning

confidence: 99%

A Study on the Interaction of the DAS‐K with Bovine Serum Albumin by On‐line Ultrafiltration and Chemiluminescence

Zhang

Zhang²,

Sun

2006

Chin. J. Chem.

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Potassium dehydroandrographolide succinate (DAS-K) has antibacterial and antiviral effects. It has been used widely for the treatment of virus pneumonia, malaria and respiratory infections. In this work, a novel flow-injection chemiluminescence (CL) method for the determination of DAS-K was proposed. The method is based on the reaction between DAS-K and hexacyanoferrate(III) in alkaline solution to give weak CL signal, which is enhanced by rhodamine B. The experimental conditions for the CL reaction were optimized and the possible reaction mechanism was discussed. Under the optimum conditions, the concentration of DAS-K is proportional to the CL intensity in the range of 0.1-80 µmol•L -1 with a detection limit of 0.05 µmol•L -1 . The interaction of the DAS-K with bovine serum albumin by on-line ultrafiltration and flow-injection chemiluminescence was studied. The concentrations of unbound DAS-K from ultra filter tube were determined by the flow-injection CL method. The binding parameters were estimated by the Scatchard plot and Klotz plot. The proposed system proved that FIA-CL coupled with on-line ultrafiltration sampling was a fast and simple technique for the study of drug-protein interaction.

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“…The flow rate of ultrafiltration was controlled by modifying both the rotation speed in the controller system and the length of the restriction. The flow rate was 5 µL min -1 , which was measured by weight [23,24]. The ultrafiltration manifold is shown schematically in Fig.…”

Section: Apparatusmentioning

confidence: 99%

“…The ultrafiltration flow rate was generated by the semi-vacuum of a Model KH-1 micropump syringe (1,000 µL) with its controller system (Institute of Biophysics, Chinese Academic of Sciences, Beijing, China) [21]. To stabilize the ultrafiltration flow, a defined capillary restriction (l=4 cm, o.d.= 0.2 mm, i.d.=0.03 mm fused silica tube) was placed in front of the syringe [23,24]. The flow rate of ultrafiltration was controlled by modifying both the rotation speed in the controller system and the length of the restriction.…”

Section: Apparatusmentioning

confidence: 99%

“…Ultrafiltration is a flexible and powerful tool for in vivo and in vitro chemistry analysis [20,21,22,23,24], which could be used as an alternative sampling method for microdialysis when coupled with detector by FIA in an on-line sampling manner. Firstly, the membrane of the ultrafiltration probe has a similar feature of molecule weight cut-off like microdialysis membrane, which allows small analytes to enter the probe together with water and ions and excludes large molecules.…”

Section: Introductionmentioning

confidence: 99%

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A flow-injection ultrafiltration sampling chemiluminescence system for on-line determination of drug?protein interaction

Wang

Zhang

et al. 2003

Analytical and Bioanalytical Chemistry

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A flow-injection ultrafiltration sampling chemiluminescence system for on-line determination of cimetidine-bovine serum albumin (BSA) interaction is proposed in this paper. Cimetidine can be oxidized by N-bromosuccinimide (NBS) and sensitized by fluorescein to produce high chemiluminescence emission in basic media. The concentration of cimetidine is linear with the CL intensity in the range 3 x 10(-7) - 1 x 10(-4) mol L(-1) with a detection limit of 1 x 10(-7) mol L(-1) (3 sigma). The drug and protein were mixed in different molar ratios in 0.067 mol L(-1) phosphate buffer, pH 7.4, and incubated at 37 degrees C in a water bath. The ultrafiltration probe was utilized to sample the mixed solution at a flow rate of 5 micro L min(-1). The data obtained by the proposed ultrafiltration flow-injection chemiluminescence method was analyzed with Scrathard analysis and a Klotz plot. The estimated association constant ( K) and the number of the binding site ( n) on one molecule of BSA by Scrathard analysis and Klotz plot were 3.15 x 10(4) L mol(-1) and 0.95, 3.25 x 10(4) L mol(-1) and 0.92, respectively. The proposed system proved that flow-injection chemiluminescence analysis coupled with on-line ultrafiltration sampling is a simple and reliable technique for the study of drug-protein interaction.

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Continuous Lactate Measurement that Combines a Portable Ultrafiltration Storage Device with an Enzyme Sensor

Cited by 7 publications

References 20 publications

Minimally Invasive Technique Based on Ultraslow Ultrafiltration To Collect and Store Time Profiles of Analytes

Minimally Invasive Technique Based on Ultraslow Ultrafiltration To Collect and Store Time Profiles of Analytes

A Study on the Interaction of the DAS‐K with Bovine Serum Albumin by On‐line Ultrafiltration and Chemiluminescence

A flow-injection ultrafiltration sampling chemiluminescence system for on-line determination of drug?protein interaction

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