Recently, cold atmospheric plasma (CAP) with the unique bio-disinfection features is used in various fields of industry, medicine, and agriculture. The main objectives of this work were to design FSG plasma (a semi-automatic device) and investigate the effect of the cold plasma in the enhancement of the Pea and Zucchini seed germination. Plasma irradiation time was studied to obtain a proper condition for the germination enhancement of seeds. The growth rate was calculated by measuring length of root and stem and dry weight of plants treated by plasma. To investigate drought resistance of plants, all treated and untreated samples were kept in darkness without water for 48 h. From the experimental results, it could be confirmed both drought resistance and germination of seedlings increased after plasma was applied to seeds at 30 s, while seeds treated whiten 60 s showed a decrease in both germination rate and seedling growth.

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The main objective of this work was to design a large-scale cold atmospheric plasma brush (LSCAPB) that can produce a perfectly homogenous large area capable of producing plasma with a length of about 5 cm for studying the inactivation effect of plasma on Gram-positive and Gram-negative bacteria that commonly implicated in Food infections. Gram-positive (L. Monocytogenes, B. Cereus) and Gram-negative (S. Typhi, V. Cholerae) bacteria with an initial population density of 1.5×108 CFU mL− 1 were treated, and their survivability with plasma exposure was examined. The effect of plasma exposure in the reduction of bacteria population was investigated using OD600 absorbance, and changes in cell structure and membrane were studied by scanning electron microscopy (SEM) analysis. Comet assay analysis was used for studying the effect of plasma exposure on DNA damage, and changes in chemical bond were analyzed by FTIR analysis. So, the best destruction is obtained at the voltage of 25 kV for all types of bacteria. This study indicates that the inactivation effect of LSCAPB correlates with the kind of bacteria. We demonstrated LSCAPB can be one of the most efficient methods for the inactivation of different types of bacteria.