INTRODUCTIONSchizophrenia is devastating psychiatric disorders characterized by positive, negative, affective and cognitive syndrome 1 which also leads to development of other co-morbid diseases.2 It disrupts mental and social functioning, destroys the lives of a patients as well as their family, friends. A number of antipsychotics have been explored over last forty years but the outcomes for patients still unsatisfactory 3 Blonanserin (BLN) a newer atypical antipsychotic belongs to a series of 4 phenyl-2-(1-piperazenyl) pyridines, chemical structure shown in Figure 1, which acts as an antagonist at dopamine D 2 , D 3 and 5HT 2A receptors. 4 With many second generation antipsychotics BLN is significantly more efficacious in the treatment of schizophrenia.5 BLN is still not official in any pharmacopoeia and based on its wide acceptability throughout world, the detail analytical profile and method of quantification of it in human plasma must be reliable, economic and highly validated. Easy, reliable, economic and accurate method of drug analysis is always well accepted, because this finally minimized the cost of dosage from 6 . Keeping the focus on worldwide huge acceptability of BLN and availability of its very limited analytical methods in plasma, present work has been planned. A details review of literature reveals that there is an availability of very few methods for the determination of BLN in human plasma and urine. Four important factors must have to consider for plasma analysis of drug during the development of new method are reasonable elution time, an appropriate LLOQ, low cost, simple and efficient sample processing. In a single HPLC method 7 where the run time of each analytes were 6 minutes which is too long. The reported UPLC/MS method 8 utilized solid phase cartridges for extraction, which is generally costly and consider complex because of further recovery of analyte from the cartridges is require, where the chance of sample loss is high. Authors also followed complex gradient elution system for the elution of analyte. One reported GS-MS method 9 uses also solid phase extraction which is considered costly because of its cartridges, and complex because of analyte recovery and selection of proper washing solution, compared to simple LLE (liquid-liquid extraction) and reported method exhibits longer analysis time. Another reported method for the determination of BLN in urine 10 found less sensitive and shows retention time 4.2 minutes and 5 minutes run time, which generally consider too long in case of LC-MS analysis. One reported method 11 used protein precipitation for the extraction of sample form the matrix shows poor peak shape and peak intensity, also exhibits long run time and retention time for the analytes. Therefore these assay methods have several significant disadvantages such as low sensitivity, complexity in the method, larger volume of sample requirement, time consuming and expensive which limits there omnipresent acceptability in clinical trial and other studies. Herein a rapid, sensit...
The present study was focused to evaluate the hypoglycemic and antidiabetic activity of Maddi Dicare the poly ‐herbal formulation, in both normal and experimentally induced hyperglycemic (Streptozotocin ) rats. Different doses of poly herbal formulation (50, 100 and 200 mg/kg) was administered in rats orally. At the end of the treatment period, the serum from normal and diabetic animals was subjected to the estimation of blood sugar, using glucose kits. Effect of Polyherbal formulation on glucose tolerance was also carried out. It was found to produce significant hypoglycemic activity in both normal and diabetic animals, which could be compared to glibenclamide (10 mg/kg). The Poly‐herbal formulation was found to increase the glucose tolerance in normal rats. The present study demonstrates that herbal formulation exhibits promising anti‐diabetic activity and helps to maintain good glycemic and metabolic control. It also proved hypoglycemic and antidiabetic activity in a dose dependent manner.
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