Pancreatic cancer is considered to be the deadliest of all cancers due to its poor prognosis and resistance to conventional therapies.

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The synthesis of stable silver nanoparticles using bioreduction method was investigated. Biological synthesis of silver nanoparticles usingTriticum aestivum(khapali ghahu) extract was investigated. The effect of a specific variety of plants and how it affects the growth of silver nanoparticles was investigated in our work and it was polydispersed. UV-visible spectroscopy was used to monitor the formation of silver nanoparticles within 15 minutes. The peaks in XRD pattern are in good agreement with those of face-centered-cubic form of metallic silver. Further the IR and TEM shows confirmation of nanocrystalline nature of silver nanoparticles. These nanoparticles dislodged by ultrasonication showed an absorption peak at 430 nm in UV-visible spectrum corresponding to the Plasmon resonance of silver nanoparticles. UV-visible titration experiments showed evidence that silver nanoparticles facilitate hydrogen peroxide reduction showing excellent catalytic activity at 200 μL. In this preliminary toxicology study, Earthworm toxicology we checked and is stable up to 1500 ppm concentration. The use of plant extract for silver nanoparticles synthesis offers the benefits of eco-friendliness and amenability for large-scale production.

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Abstract:A new sensitive sensor based on graphene quantum dots was constructed and used to detect gene mutations in pancreatic cancer (PC). PC is a leading cause of death worldwide and is considered a hard-to-treat malignancy due to its late diagnosis and lowest 5-year survival rate of 2%-7%. Despite significant advances in detection techniques, PC is often diagnosed at advanced stage, with limited treatment options available, which leads to death in the majority of cases. In this regard, it is extremely important to develop methods for early detection of this lethal disease. In this study, we explored the advantages of electrochemical biosensing and designed a simple screen-printed DNA sensor for the detection of gene mutation in PC. p16 is a tumor-suppressor gene, and alterations in the gene sequence are related to PC onset. Therefore, we explored the p16 gene as a cancer marker and designed an electrochemical on-chip sensor system for the early diagnosis of PC. Different electrochemical techniques, such as differential pulse voltammetry and electrochemical impedance spectroscopy, were applied along with other characterization techniques (eg, ultraviolet-visible spectroscopy, scanning and transmission electron microscopy) for designing a simple DNA hybridization sensor for PC. The biosensor showed a detection capability of 0.10 pM. This study establishes the utilization of graphene quantum dots as a sensor matrix and a new approach for detecting gene mutation on a chip, which can be further developed as an integrated portable detection system.