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In this paper the antimicrobial activity of the Water extract of Gymnema sylvestre fruit and root were studied. Antimicrobial studies showed that the extract has considerable activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes and Aspergillus niger.

The Metal content of Gymnema sylvestre medicinal plant was determined by using Xray Fluorescence Spectroscopy (XRF). The leaf /stem/root has the highest concentration of metals like Ca, K, Si, Mg, P and Al. The concentration of Ca (45690ppm) in root is higher than leaf (35640 ppm) and stem (31330 ppm). The amount of K is 24060 ppm, 25320 ppm and 11180 ppm in leaf, stem and Root respectively. The G. Sylvestre also contains metals like Si and Mg in higher amount. The results obtained in this study suggest that the use of this plant can be beneficial to the users in cases of nutrient deficiency. The information provided in this study could be helpful in standardization of herbal drugs since the Gymnema sylvestre medicinal plants play an important role in maintenance diabetes of Type I and II.