Chemical Examination and Evaluation of Antioxidant and Antimicrobial Activities of Essential Oil from<i>Gymnema sylvestre</i>R. Br. Leaves

Naik, D. G.; Dandge, Chitra; Rupanar, Shobha

doi:10.1080/10412905.2011.9700451

Cited by 23 publications

(12 citation statements)

References 39 publications

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“…Further confirmation of assignments was done by Co-GC analyses with known standards of available authentic compounds. Quantification of each constituent was done by following the standard procedure reported in our earlier paper (Naik et al 2011).…”

Section: Methodsmentioning

confidence: 99%

Potential Use of an Essential Oil From the Flower of Swertia densifolia as a Repellent for Apis florea (Hymenoptera: Apidae)

Naik¹,

Vaidya-Kannur²,

Deshpande³

et al. 2015

Annals of the Entomological Society of America

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Formulations of compounds repellent to honey bees may be useful tools to keep bees away from areas recently treated with toxic insecticides. Here, we report the activity of a previously unknown honey bee repellent, an essential oil from the flower of Swertia densifolia (Gentianaceae), which has activity toward Indian honey bees Apis florea F. The essential oil was obtained by hydrodistillation and was subjected to a rotating table bioassay with foragers of the honey bee A. florea. Repellency was dose-dependent up to 12 mg/ml and remained constant thereafter. The chemical constitution of the essential oil was examined by gas chromatography-mass spectrometry analyses, which identified 19 constituent compounds. The major constituents (>10% of the oil) were linalool and octadecanoic acid. The constituents of intermediate concentration (5-10%) were a-terpeniol, nerol, n-octadecyl acetate, and spirostan-3-ol. Besides these, five minor constituents (1-5%) and six of the eight trace components present (<1%) have also been identified. The response of A. florea foragers to mixtures of the major and intermediate constituents was studied. Screening of formulations of linalool and a-terpeniol showed repellent properties toward A. florea, behavior which has already been reported by us in earlier studies. The compound n-octadecyl acetate was found to be an attractant, while octadecanoic acid and spirostan-3-ol were neither attractants nor repellents. Nerol, however, elicited dose-dependent effects involving both attraction and repellency. The effects of the constituents of this essential oil were not merely additive, possibly due to synergistic effects. These results establish the previously unknown chemical constitution and dose-dependent repellent nature of the essential oil isolated from flower of S. densifolia toward A. florea and may be used to improve bee management.

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Section: Methodsmentioning

confidence: 99%

Potential Use of an Essential Oil From the Flower of Swertia densifolia as a Repellent for Apis florea (Hymenoptera: Apidae)

Naik¹,

Vaidya-Kannur²,

Deshpande³

et al. 2015

Annals of the Entomological Society of America

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“…The assay was carried out by reported procedure 34 . A solution (0.025 mm) of DPPH in methanol was prepared.…”

Section: Dpph Radical Scavenging Assay By Ic 50 Methodsmentioning

confidence: 99%

Phytochemical Screening and In Vitro Antioxidant Studies of Fagara Budrunga (Zanthozylum Rhesta) Fruit Extracts

2016

Chem Sci Trans

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Fruits of Fagara budrunga was extracted with solvents of different polarities. Antioxidant activity of n-hexane, ethyl acetate and methanol extracts were determined in vitro by DPPH free radical scavenging assay, β-carotene bleaching assay and reducing power assays. BHT was used as the standard. The radical scavenging activity and reducing power of the extracts significantly decreased in the order methanol extract > ethyl acetate extract > n-hexane extract. A strong inhibition of lipid peroxidation in β-carotene bleaching assay was exhibited by n-hexane extract followed by ethyl acetate extract and methanol extract. The methanol extract was found to possess highest phenolic content (75 mg GAE/g dry weight) among the extracts. It is seen that the yield and activities of extract was affected by the solvent used for extraction. Phytochemical investigation of the extracts indicated the presence of alkaloids, flavonoids, tannins, steroids and terpenoids. The present study provides phytochemical screening results and evaluation of antioxidant activities of F. budrunga extracts.

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“…Quantitative analysis: Calibration curve of each of the standard compounds, β-pinene, eucalyptol, 2-Dodecanone and eugenol was generated using six concentration points. The response factor of the respective standard and the percentage of each chemical constituent in the essential oil were determined by following the reported procedure [52,53].…”

Section: Determination Of Chemical Compositionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Different Chemotype Expression Profile Analysis of Genes Related to Monoterpenoid Biosynthesis in Cinnamomum porrectum

Qiu

Wang

Zheng

et al. 2019

IJMS

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Leaves of C. porrectum are rich in essential oils containing monoterpenes, sesquiterpenes and aromatic compounds, but the molecular mechanism of terpenoid biosynthesis in C. porrectum is still unclear. In this paper, the differences in the contents and compositions of terpenoids among three chemotypes were analyzed using gas chromatography mass spectrometry (GC/MS). Furthermore, the differential expression of gene transcripts in the leaf tissues of the three C. porrectum chemotypes were analyzed through a comparison of full-length transcriptomes and expression profiles. The essential oil of the three C. porrectum chemotypes leaves was mainly composed of monoterpenes. In the full-length transcriptome of C. porrectum, 104,062 transcripts with 306,337,921 total bp, an average length of 2944 bp, and an N50 length of 5449 bp, were obtained and 94025 transcripts were annotated. In the eucalyptol and linalool chemotype, the camphor and eucalyptol chemotype, and the camphor and linalool chemotype comparison groups, 21, 22 and 18 terpene synthase (TPS) unigenes were identified respectively. Three monoterpene synthase genes, CpTPS3, CpTPS5 and CpTPS9, were upregulated in the eucalyptol chemotype compared to the linalool chemotype and camphor chemotype. CpTPS1 was upregulated in the camphor chemotype compared to the linalool chemotype and the eucalyptol chemotype. CpTPS4 was upregulated in the linalool chemotype compared to the camphor chemotype and the eucalyptol chemotype. Different unigenes had different expression levels among the three chemotypes, but the unigene expression levels of the 2-C-methyl-D-erythritol 4phosphate (MEP) pathway were generally higher than those of the mevalonate acid (MVA) pathway. Quantitative reverse transcription PCR(qRT-PCR) further validated these expression levels. The present study provides new clues for the functional exploration of the terpenoid synthesis mechanism and key genes in different chemotypes of C. porrectum.

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Chemical Examination and Evaluation of Antioxidant and Antimicrobial Activities of Essential Oil fromGymnema sylvestreR. Br. Leaves

Cited by 23 publications

References 39 publications

Potential Use of an Essential Oil From the Flower of Swertia densifolia as a Repellent for Apis florea (Hymenoptera: Apidae)

Potential Use of an Essential Oil From the Flower of Swertia densifolia as a Repellent for Apis florea (Hymenoptera: Apidae)

Phytochemical Screening and In Vitro Antioxidant Studies of Fagara Budrunga (Zanthozylum Rhesta) Fruit Extracts

Full-Length Transcriptome Sequencing and Different Chemotype Expression Profile Analysis of Genes Related to Monoterpenoid Biosynthesis in Cinnamomum porrectum

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