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Recently, cloud point extraction (CPE) coupled with back extraction (BE) has been suggested as a promising alternative to liquid-liquid extraction. In CPE, non-ionic surfactants in aqueous solutions form micelles and the solution becomes turbid when heated to the cloud point temperature. Microwave- or ultrasonic-assisted BE can be performed after CPE and before injection of the sample for instrumental analysis by ultraviolet-visible spectroscopy, high-performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, or liquid chromatography-mass spectrometry. This article reviews selected published scientific research on the application of CPE-BE to the determination of alkaloids, drugs and organophosphorus compounds from several complex matrices. This method could be scaled-up for use in forensic science.

In this short communication, various causes of breast cancer have been illustrated. Breast cancer is a one of the serious disease in women in world wide. Increasing age, reproductive factors, mammographic density and, in around 9% of cases, genetic factors and family history are all known risk factors for breast cancer. A range of factors, such as dietary fat intake, and solvent and pesticide exposure, have also been implicated as potential risk factors but the evidence so far has been inconclusive. For most cases of breast cancer, the true cause remains unknown. Studies worldwide have identified many factors to which women with breast cancer attribute their condition, including stress and other psychosocial factors; knocks, bruises or injury to the breast; religious causes; chemicals, food additives; proximity to electronic equipment or overhead power lines; viral or bacterial infection; and bad luck, although there is limited evidence to support the attributions. The objective of this study was too aware the women from various causes of breast cancer.

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A green, simple and cost-effective, validated high-performance liquid chromatographic (HPLC) with UV detector has been developed an analytical method for the simultaneous determination of the most significant phenolic compounds viz. Gallic acid (GA), Quercetin (QC) and Ferulic acid (FA). The detection limit of GA, QC, FA was 0.303, 0.168, 0.0976 μg/mL and quantitation limit was 0.918, 0.051, 0.295 μg/mL, respectively. All parameters of planned validated method were carried out as per the ICH guidelines. This analytical method was applied to determine the phenolic compounds in fruits cultivated in India. Phenolic compounds in fruits extract were quantified using regression equations. This method can be scaled up to determine the alkaloids from several medicinal and poisonous plants samples in pharmaceutical, forensics and industrial areas.