Olfactory dysfunction is one of the
most frequent and specific
symptoms of coronavirus disease 2019 (COVID-19). Information on the
damage and repair of the neuroepithelium and its impact on olfactory
function after COVID-19 is still incomplete. While severe acute respiratory
syndrome coronavirus-2 (SARS-CoV-2) causes the ongoing worldwide outbreak
of COVID-19, little is known about the changes triggered by SARS-CoV-2
in the olfactory epithelium (OE) at the cellular level. Here, we report
profiles of the OE after SARS-CoV-2 infection in golden Syrian hamsters,
which is a reliable animal model of COVID-19. We observed severe damage
in the OE as early as 3 days postinoculation and regionally specific
damage and regeneration of the OE within the nasal cavity; the nasal
septal region demonstrated the fastest recovery compared to other
regions in the nasal turbinates. These findings suggest that anosmia
related to SARS-CoV-2 infection may be fully reversible.
Hearing loss is the most frequent sensory disorder in humans. Auditory hair cells (HCs) are postmitotic at late-embryonic differentiation and postnatal stages, and their damage is the major cause of hearing loss. There is no measurable HC regeneration in the mammalian cochlea, and the maintenance of cell function is crucial for preservation of hearing. Here we generated mice deficient in autophagy-related 5 (Atg5), a gene essential for autophagy, in the HCs to investigate the effect of basal autophagy on hearing acuity. Deletion of Atg5 resulted in HC degeneration and profound congenital hearing loss. In autophagy-deficient HCs, polyubiquitinated proteins and p62/SQSTM1, an autophagy substrate, accumulated as inclusion bodies during the first postnatal week, and these aggregates increased in number. These findings revealed that basal autophagy has an important role in maintenance of HC morphology and hearing acuity.
Lassa virus (LASV) is the causative agent of Lassa fever (LF), which presents as a lethal hemorrhagic disease in severe cases. LASV-induced hearing loss in survivors is a huge socioeconomic burden, however, the mechanism(s) leading to hearing loss is unknown. In this study, we evaluate in a mouse LF model the auditory function using auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) to determine the mechanisms underlying LASV-induced hearing loss. In the process, we pioneered measures of ABR and DPOAE tests in rodents in biosafety level 4 (BSL-4) facilities. Our T cell depletion studies demonstrated that CD4 T-cells play an important role in LASV-induced hearing loss, while CD8 T-cells are critical for the pathogenicity in the acute phase of LASV infection. Results presented in this study may help to develop future countermeasures against acute disease and LASV-induced hearing loss.
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