The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34°C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27°C and 33°C and then at 27°C (33-27°C). This strain showed little growth at higher than 35°C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34°C, and only the cells at 34°C revealed a failure of cytokinesis. The structure of the mannans revealed by 1 H-NMR analysis that the mannans obtained at both 33 and 34°C had drastically decreased two consecutive b b-1,2-linked mannopyranose units at the nonreducing terminal of the a a-linked oligosaccharides and increased one b b-1,2-linked mannopyranose unit at the nonreducing terminal attached to the a a-1,3-linked mannose unit and the non-reducing terminal a a-1,3-and a a-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34°C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit 'Candida Check'.
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