Structural Changes of Cell Wall Mannans of Candida guilliermondii IFO 10279 Strain Cells Cultured at High Temperature

Abstract

The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34°C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27°C and 33°C and then at 27°C (33-27°C). This strain showed little growth at higher than 35°C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34°C, and only the cells at 34°C revealed a failure of cytokinesis. The structure of the mannans revealed by 1 H-NMR analysis that the mannans obtained at both 33 and 34°C had drastically decreased two consecutive b b-1,2-linked mannopyranose units at the nonreducing terminal of the a a-linked oligosaccharides and increased one b b-1,2-linked mannopyranose unit at the nonreducing terminal attached to the a a-1,3-linked mannose unit and the non-reducing terminal a a-1,3-and a a-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34°C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit 'Candida Check'.

Coronary Vasculitis Induced in Mice by Cell Wall Mannoprotein Fractions of Clinically Isolated <i>Candida</i> Species

“…We previously reported that CAWS727, prepared from the culture supernatant of C. albicans NBRC1385 cultured at 27ºC for 2 days in pH-controlled C-limiting medium (pH 7), did not induce vasculitis, and this was attributed to the presence of β-mannosyl linkages 11) . These findings were strongly supported by Shibata et al, who reported that βmannosyl transferase was only active at approximately 27ºC and neutral pH 57,58,61) . In the present study, we compared the structures of MN fractions using NMR analysis and found that signals assignable to β -mannosyl linkages were detected in various species, including C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, a n d C. tropicalis 16,27,28,36,37,42,57,58,60,[62][63][64][65] .…”

“…5, the spectra of MN fractions in each species were similar to each other and showed species-specific signal patterns. According to Shibata's assignment, the anomeric signals of α -mannose and βmannose residues were characteristic to species and appeared at approximately 5.6-4.9 and 5.0-4.7 27,42,[57][58][59] . Consistent with these results, all of the spectra contained signals assignable to α-mannan.…”

Disruption of Protein Mannosylation Affects Candida guilliermondii Cell Wall, Immune Sensing, and Virulence

“…C. guilliermondii cell wall contains chitin, which increases in amount in response to exposure to sub-lethal concentrations of caspofungin (Walker et al, 2013). Furthermore, structural analysis of C. guilliermondii cell wall mannans indicated the presence of α1,2-, α1,3- and β1,2-mannose units, suggesting a similar organization to the C. albicans mannans (Okawa et al, 2006). …”

Beta-Mannosyl Linkages Negatively Regulate Anaphylaxis and Vasculitis in Mice, Induced by CAWS, Fungal PAMPs Composed of Mannoprotein-Beta-Glucan Complex Secreted by Candida albicans

“…Regulation of beta-1,2-mannosyl linkages by culture condition is also reported in Candida guilliermondii. 23) From the data of immunochemistry and NMR spectra, CAWS 27-5.2 and CAWS 27-7.0 contained significantly higher concentrations of beta-1,2-linked mannooligosaccharides.…”