No abstract

This study was conducted to explore specific chill models and the mechanisms underlying rhizome bud dormancy break in Polygonatum kingianum. Rhizome buds were subjected to various chilling temperatures for different duration and then transferred to warm conditions for germination and subsequent evaluation of their response to temperature and chilling requirements. A CU kingianum model was constructed to describe the contribution of low temperature to the chill unit, and it was suggested that 2.97˚C was the optimum temperature and that 11.54˚C was the upper limit for bud release. The CAS kingianum model showed the relationship between chilling accumulation and sprouting percentage; therefore, rhizome bud development could be predicted through the model. Weighted correlation network analysis (WGCNA) of transcriptomic data of endo-, eco-and nondormant rhizome buds generated 33 gene modules, 6 of which were significantly related to bud sprouting percentage. In addition, 7 significantly matched transcription factors (TFs) were identified from the promoters of 17 "real" hub genes, and DAG2 was the best matched TF that bound to AAAG element to regulate gene expression. The current study is valuable for developing a highly efficient strategy for seedling cultivation and provides strong candidates for key genes related to rhizome bud dormancy in P. kingianum.

Two-dimensional (2D) triaxial braided composites with braiding angle (± 60°/0°) have been used as aero-engine containing casing material. In the current paper, three types of projectile with the same mass and equivalent diameter, including cylinder gelatin projectile, carbon fiber-reinforced plastics (CFRP), and titanium alloy blade-like projectile, were employed to impact on triaxial braided composites panels with thickness of 4.3 mm at room temperature (20 °C) to figure out the influences of projectile materials on the damage pattern and energy absorption behavior. Furthermore, the influences of environmental temperature were also discussed considering the aviation service condition by conducting ballistic impact tests using CFRP projectile at cryogenic temperature (−50 °C) and high temperature (150 °C). The triaxial braided target panel were pre-heated or cooled in a low-temperature chamber before mounted. It is found that soft gelatin project mainly causes global deformation of the target and therefore absorb much more energy. The triaxial braided composite absorb 77.59% more energy when impacted with CFRP projectile than that with titanium alloy projectile, which mainly results in shear fracture. The environmental temperature has influences on the damage pattern and energy absorption of triaxial braided composites. The cryogenic temperature deteriorates the impact resistance of the triaxial braided composite material with matrix cracking damage pattern, while high temperature condition improves its impact resistance with shearing fracture damage pattern.