PurposeWe investigated whether recombinant human granulocyte colony-stimulating factor (rhG-CSF) could promote the development of preinvasive and invasive breast cancer in mouse mammary tumor virus (MMTV-erbB2) mice with estrogen receptor-positive tumors.MethodsMMTV-erbB2 mice were randomly divided into three experimental groups with 20 mice in each group. MMTV-erbB2 mice were treated with daily subcutaneous injections of vehicle or rhG-CSF (low-rhG-CSF group, rhG-CSF 0.125 µg; vehicle-rhG-CSF group, normal saline 0.25 µg; and high-rhG-CSF group, rhG-CSF 0.25 µg) at 3 months of age. Cellular and molecular mechanisms of G-CSF action in mammary glands were investigated via immunohistochemistry and reverse transcription polymerase chain reaction.ResultsLow, but not high, rhG-CSF doses significantly accelerated mammary tumorigenesis in MMTV-erbB2 mice. Short-term treatment with rhG-CSF could significantly promote the development of preinvasive mammary lesions. The cancer prevention effect was associated with reduced expression of proliferating cell nuclear antigen, cluster of differentiation 34, and signal transducers and activators of transcription 3 in mammary glands by >80%.ConclusionWe found that G-CSF was regulated by rhG-CSF both in vitro and in vivo. Identification of G-CSF genes helped us further understand the mechanism by which G-CSF promotes cancer. Low doses of rhG-CSF could significantly increase tumor latency and increase tumor multiplicity and burden. Moreover, rhG-CSF effectively promotes development of both malignant and premalignant mammary lesions in MMTV-erbB2 mice.
The technology of using diisopropylamine as a replaced drying solvent preparing for nano material has been studied.The products of Al(OH)3 and SiO2 are found to be amorphous aerogel
The technology of producing nano material by diisopropylamine as a replaced drying solvent has been studied.The products of Al(OH)3 and SiO2 are found to be amorphous aerogel.The nano particle of TiO2 produced by it is found to be smaller than that produced by n-butyl alcohol as a replaced drying Solvent.
The technology of synthesis nanomaterial by diisopropylamine as a replaced drying solvent has been studied.The products of Al (OH)3 and SiO2 are found to be amorphous aerogel.The nanoparticle of TiO2 produced by it is found to be smaller than that produced by n-butyl alcohol as a replaced drying Solvent.
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