Study on the Nano Material Produced by Diisopropylamine or N Butyl Alcohol as Exchange Solvent

The preparation, approximate composition, degree of hydrolysis (DH), lipase activity and antioxidant capacity (including the ability of scavenging DPPH and ABTS free radicals) were determined for various of aqueous enzymatic extract (AEE) obtained from rice bran (RB) using alcalase (A), trypsin (T), papain (Pn), neutrase (N) and protamex (Px). Peptides and γ-oryzanol are the major functional components of AEE. Generally, the protein yield varied with the type of enzyme used in the order of T>Pn>N>Px>A. Both trypsin and alcalase extracts exhibit a higher level of γ-oryzanol than other enzymes. Lipase activity was measured using the 4-Nitrophenyl Laurate (p-NPL) substrate method, and significantly different (P<0.05) lipase activity values ranging from 0.55 to 2.38 mU/mL were observed from five hydrolysates. Furthermore, two synthetic free radicals were used to evaluate the antioxidant activity of AEE. The alcalase extract (at 4.35±0.15mg protein/mL AEE) shows the greatest scavenging rate (28.42%) of DPPH, but trypsin extract has highest ABTS radical-scavenging activity of 94.05%. Data revealed trypsin was possible the most effective extractant under their optimal conditions. These results demonstrate that the product obtained from rice bran by different enzymatic digestions may contain bioactive components and might potentially be natural antioxidants. Meanwhile, the aqueous enzymatic extraction method can also improve the stability of the hydrolysates.

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“…DH (%) was determined for each digestion by assaying free amino groups with pH-stat using the below Eq. ( 1) [14]:…”

Section: Methodsmentioning

confidence: 99%

Aqueous Enzymatic Method for Preparing the Mixed Extract with Antioxidant Activity from Rice Bran

Sun

Guo

Wang

et al. 2013

AMR

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Study on the Nano Material Produced by Diisopropylamine or N Butyl Alcohol as Exchange Solvent

Cited by 1 publication

References 2 publications

Aqueous Enzymatic Method for Preparing the Mixed Extract with Antioxidant Activity from Rice Bran

Aqueous Enzymatic Method for Preparing the Mixed Extract with Antioxidant Activity from Rice Bran

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