The present investigation is aimed to conduct a comparative study that examine the effect of pranayama on premenstrual syndrome to gain insight on the role of pranayama. Total 100 females age range from 10 to 50 years participated in this study from Kapurthala, district of Punjab, India. Further the population was divided into two groups with equal number of participants (n=50), group A (no intervention) and group B (pranayama). The subjects of group A did not perform any type of breathing and yogic technique for past four to five months and group B used to perform pranayama exercise on regular basis. Results showed positive significant effect of pranayama practices on premenstrual syndrome (t=58.26, p<.01).