Malignant glioma is a common cancer of the nervous system. Despite recent research efforts in cancer therapy, the prognosis of patients with malignant glioma has remained dismal. MicroRNAs are noncoding RNAs that inhibit the expression of their targets in a sequence-specific manner, and a few have been shown to act as oncogenes or tumor suppressors. Here, we aimed at exploring the precise biological role of microRNA-7 (miR-7) and the global protein changes in glioma cell lines transiently transfected with miR-7. Transfection of miR-7 into glioma cell lines causes inhibition of cell migration and invasion and suppression of tumorigenesis. Moreover, ectopic expression of miR-7 inhibits lung metastases of glioma in vivo. Among 65 protein spots with differential expression separated by 2-DE, 37 proteins were successfully identified by MS/MS analysis. Of those, the 25 downregulated proteins, which include 14-3-3ζ, eukaryotic translation initiation factor 5A (EIF5A), and annexin A4, may be downstream targets of miR-7, a finding that could elucidate some aspects of the behavior of glioma cells at the protein level. In conclusion, the absence of miR-7 function could cause downstream molecules to switch on or off, resulting in glioma development, invasion, and metastases. MiR-7-based gene treatment may be a novel anti-invasion therapeutic strategy for malignant glioma.
In the existing biomass briquette production market, the screw extrusion-type machines are applied at the earliest. However, the shortcoming of the easily-worn screw head limits their application and large-scale development. In this paper, the main reason of the impact on screw life can be identified by the force and motion analysis of molding biomass. Therefore, theoretical basis for the improvement and optimized design of the molding machine can be provided. Furthermore, new paths which can increase the machine efficiency, reduce production costs and increase biomass briquette production can be found.
In order to analyze the content of fatty acid methyl esters, biodiesel was analyzed by gas chromatography method. Biodiesel was produced from the raw materials soybean oil and mussel shell catalysts. GC analysis was developed by using HP-innowax chromatographic column and FID detector. Undecanoic acid methyl ester was chosen as internal standard solution. The results show that five kinds of fatty acid methyl esters were linear at range 4 g•L-1~31 g•L-1 (γ≥0.9928). The average recovery rate was 98.28%~101.85%, and relative standard deviation was less than 0.31%. The coefficient of variance of precision was less than 1.59%. This GC method is simple, rapid and accurate and it will be the base for further research.
Calcium dioxide can catalyze soybean oil to obtain biodiesel in triglyceride transesterification. Waste mussel shells were utilized as a source of calcium oxide. The shell was calcined in N2 at 1000°C with optimum time of 2 h to obtain active CaO catalysts. Parametric studies have been investigated and perfect conditions were found to be methanol/oil mole ratio, 9:1; catalyst amount, 4wt%; reaction temperature, 65°C; stirring rate of 450 round per minute; reaction time, constant 3h; methyl esters conversion above 98% . Reusability results improved that the prepared catalyst is able to be reemployed up to 9 times. These results indicate waste mussel shell combusted at high temperature can be reused in biodiesel production as a low cost catalyst.
The state of charge (SOC) is an important index for power battery system. To obtain its accurate value,a comprehensive equivalent circuit model that parameters change depend on SOC was estiblished in this paper by using the lithium-ion battery hybrid pulse power characteristic data. Then the Extended Kalman filter (EKF) method is applied to estimate the SOC under the working condition. Numerical simulations are conducted to verify the effectiveness of the model and the EKF method. The results show that the EKF method based on the dynamic model can satisfy the accuray requirements.
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