The effect of miR‐7 on behavior and global protein expression in glioma cell lines

Lu, Ze; Liu, Sheng Yong; Yao, Yu; Zhou, Yong; Zhang, Shuang; Dai, Lei; Tian, Hong; Zhou, Yan; Deng, Hong; Yang, Jin; Luo, Feng

doi:10.1002/elps.201100230

Cited by 24 publications

(19 citation statements)

References 32 publications

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“…The expression of Anxa4 was inhibited by miR-7 overexpression in glioma cells, miR-7 overexpression inhibited migration and invasion capacities of glioma cell lines U251, U87, and A172 reduced the tumorigenicity and metastasis of U251 cells in nude mice [71]. Proteomic analysis demonstrated that Anxa4 was decreased 3.5-fold in miR-7 overexpressed U251 cells compared with control group [71].…”

Section: Anxa4 and Gliomamentioning

confidence: 95%

Annexin A4 and cancer

Wei

Guo

Liu

et al. 2015

Clinica Chimica Acta

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Section: Anxa4 and Gliomamentioning

confidence: 95%

Annexin A4 and cancer

Wei

Guo

Liu

et al. 2015

Clinica Chimica Acta

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“…MicroRNAs (miRNAs) that regulate 14-3-3ζ have been reported in the literature. In glioma cells, the over-expression of microRNA-7 (miR-7) down-regulated 14-3-3ζ [14]. In K562 cells, up-regulated expression of microRNA-375 (miR-375) was associated with down-regulated expression of 14-3-3ζ [15].…”

Section: Introductionmentioning

confidence: 99%

Altered Expression of 14-3-3ζ Protein in Spinal Cords of Rat Fetuses with Spina Bifida Aperta

Wei

Yang

et al. 2013

PLoS ONE

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BackgroundA large number of studies have confirmed that excessive apoptosis is one of the reasons for deficient neuronal function in neural tube defects (NTDs). A previous study from our laboratory used 2-D gel electrophoresis to demonstrate that 14-3-3ζ expression was low in the spinal cords of rat fetuses with spina bifida aperta at embryonic day (E) 17. As a member of the 14-3-3 protein family, 14-3-3ζ plays a crucial role in the determination of cell fate and anti-apoptotic activity. However, neither the expression of 14-3-3ζ in defective spinal cords, nor the correlation between 14-3-3ζ and excessive apoptosis in NTDs has been fully confirmed.Methodology/Principal FindingsWe used immunoblotting and quantitative real-time PCR (qRT-PCR) to quantify the expression of 14-3-3ζ and double immunofluorescence to visualize 14-3-3ζ and apoptosis. We found that, compared with controls, 14-3-3ζ was down-regulated in spina bifida between E12 and E15. Excessive apoptotic cells and low expression of 14-3-3ζ were observed in the dorsal region of spinal cords with spina bifida during the same time period. To initially explore the molecular mechanisms of apoptosis in NTDs, we investigated the expression of microRNA-7 (miR-7), microRNA-375 (miR-375) and microRNA-451 (miR-451), which are known to down-regulate 14-3-3ζ in several different cell types. We also investigated the expression of p53, a molecule that is downstream of 14-3-3ζ and can be down-regulated by it. We discovered that, in contrast to the reduction of 14-3-3ζ expression, the expression of miR-451, miR-375 and p53 increased in spina bifida rat fetuses.Conclusions/SignificanceThese data suggest that the reduced expression of 14-3-3ζ plays a role in the excessive apoptosis that occurs in spina bifida and may be partly regulated by the over-expression of miR-451 and miR-375, and the consequent up-regulation of p53 might further promote apoptosis in spina bifida.

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“…2-DE was performed as described previously [8]. Briefly, cells were lysed in lysis buffer (8M urea, 2M thiourea, 4% CHAPS, 100mM DTT, 0.2% pH 3-10 ampholyte, BioRad, Hercules, CA) containing 1% protease inhibitor cocktail 8340 (104 mM AEBSF, 80 μM Aprotinin, 4 mM Bestatin, 1.4 mM E-64, 2 mM Leupeptin, 1.5mM Pepstatin A) (Sigma, St Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomic Analysis of Anti-Cancer Mechanism by Periplocin Treatment in Lung Cancer Cells

Song

Yang

et al. 2014

Cell Physiol Biochem

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Background: Periplocin is used for treatment of rheumatoid arthritis, reinforcement of bones and tendons, palpitations or shortness of breath and lower extremity edema in traditional medicine. Our previous findings suggested that periplocin could inhibit the growth of lung cancer both in vitro and in vivo. But the biological processes and molecular pathways by which periplocin induces these beneficial effects remain largely undefined. Methods: To explore the molecular mechanisms of periplocin involved in anti-cancer activity, in the present study the protein profile changes of human lung cancer cell lines A549 in response to periplocin treatment were investigated using the proteomics approaches (2-DE combined with MS/MS). Western blot was employed to verify the changed proteins. Interactions between changed proteins were analyzed by STRING. Results: 29 down-regulated protein species named GTP-binding nuclear protein Ran (RAN), Rho GDP-dissociation inhibitor 1 (ARHGDIA), eukaryotic translation initiation factor 5A-1 (EIF5A) and Profilin-1(PFN1), and 10 up-regulated protein species named Heat shock cognate 71 kDa protein (HSPA8),10 kDa heat shock protein (HSPE1), and Cofilin-1(CFL-1) were identified. Among them, GTP-binding nuclear protein Ran (RAN) and Rho GDP-dissociation inhibitor 1 (ARHGDIA) were the most significantly changed (over tenfold). The proteasome subunit beta type-6 (PSMB6), ATP synthase ecto-α-subunit (ATP5A1), Aldehyde dehydrogenase 1 (ALDH1) and EIF5A were verified by immunoblot assays to be dramatically down-regulated. By STRING bioinformatics analysis revealing interactions and signaling networks it became apparent that the proteins changed they are primarily involved in transcription and proteolysis. Conclusion: Periplocin inhibited growth of lung cancer by down-regulating proteins, such as ATP5A1, EIF5A, ALDH1 and PSMB6. These findings may improve our understanding of the molecular mechanisms underlying the anti-cancer effects of periplocin on lung cancer cells.

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The effect of miR‐7 on behavior and global protein expression in glioma cell lines

Cited by 24 publications

References 32 publications

Annexin A4 and cancer

Annexin A4 and cancer

Altered Expression of 14-3-3ζ Protein in Spinal Cords of Rat Fetuses with Spina Bifida Aperta

Comparative Proteomic Analysis of Anti-Cancer Mechanism by Periplocin Treatment in Lung Cancer Cells

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