Objective: The aim of this study is to analyze the phytochemical constituents and the in vitro immunomodulatory potential of ethanol extract of Nigella sativa and Psoralea corylifolia seeds.Methods: Phytoconstituents in the ethanol extract of N. sativa and P. corylifolia seeds were analyzed using gas chromatography and mass spectrum and their immunomodulatory activity was investigated in vitro by cell proliferation assay with J774A.1 cell line.Results: Thirteen biochemical constituents have been identified with ethanol extract of N. sativa and nine from the P. corylifolia. The maximum relative activity of 138.77±0.44% cell proliferation was expressed at 25 μg/ml by N. sativa and 80.70±0.42% of proliferation by P. corylifolia at 25 μg/ml.
Conclusion:The results suggest that the ethanol extract of the plant seeds have stimulating activity on macrophage cells and could be useful for modulating immune functions.
The medicinal plants have played a vital role in drug discovery. Many plants are used traditionally for the treatment of different types of viral disorders and other infectious diseases. Herpes simplex virus (HSV) is a ubiquitous organism that causes infections in human populations throughout the world. It causes a variety of diseases ranging in severity from mild to life-threatening. The present study was to evaluate antiviral activity of Hypericum mysorense F. Heyne ethanolic leaf extract against herpes simplex virus type 2 (HSV-2) using HEp-2 cell line. The toxic free concentrations of ethanolic extract were determined using MTT assay, followed by anti HSV-2 activity and a positive control was maintained. 50?g/ml of ethanol extract was observed to be maximum non toxic free concentration against HEp-2 cell line and also exhibited significant inhibitory activity against HSV-2.
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