A psychrophilic, Gram-negative bacterium, designated MOL-1 T , was isolated from water of Lake . The major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 v7c). Menaquinone MK-7 is the predominant respiratory quinone, while symhomospermidine is the predominant polyamine. The polar lipid profile is composed of the predominant lipids phosphatidylethanolamine and unidentified polar lipid L2, with moderate amounts of unidentified polar lipids L1, L5 and L6 and unidentified aminophospholipids APL1 and APL2 and minor to trace amounts of unidentified polar lipids L3, L4, L7, L8, L9 and L10, unidentified phospholipid PL4 and unidentified aminophospholipid APL3. After molecular and phenotypic studies, including chemotaxonomic analyses, it was concluded that strain MOL-1 T represents a novel Sphingobacterium species, for which the name Sphingobacterium psychroaquaticum sp. nov. is proposed. The type strain is MOL-1 T (5NRRL B-59232 T 5DSM T).

Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys Two facultatively anaerobic, budding bacterial strains, designated W1215-PCA4 T and SRNK-1, were isolated from water from Lake Michigan, USA. The two strains showed identical ERIC-PCRgenerated genomic fingerprints and shared 99.9 % 16S rRNA gene sequence similarity. Strain W1215-PCA4 T showed highest 16S rRNA gene sequence similarities to Labrys monachus VKM B-1479 T (95.8 %), Labrys methylaminiphilus DSM 16812 T (95.1 %), Labrys okinawensis MAFF 210191 T (96.0 %), Labrys miyagiensis G24103 T (95.4 %), Labrys neptuniae BCRC 17578 T (95.7 %) and Labrys portucalensis DSM 17916 T (95.8 %). Data suggested that the two strains were members of a single novel species of the genus Labrys. The major cellular fatty acids of the two isolates were C 18 : 1 v7c, C 19 : 0 cyclo v8c and C 16 : 0 . Their polar lipid profiles were highly similar to that of Labrys monachus DSM 5896 T . The primary quinone was ubiquinone Q-10, with minor amounts of Q-9 and Q-11. sym-Homospermidine was the predominant polyamine, with putrescine present in moderate amounts. The two strains were identical in terms of their biochemical and physiological traits, but were distinguishable from other species of the genus Labrys. Hence, the description of a novel species in this genus appears to be justified. The name Labrys wisconsinensis sp. nov. is proposed; the type strain is W1215-PCA4 T (5DSM 19619 T 5NRRL B-51088 T ).

Three strains of a Gram-stain negative bacterium were isolated from Lake Michigan water. 16S rRNA gene sequence analysis revealed that strain 1131 had sequence similarities to Bosea vaviloviae LMG 28367T, Bosea lathyri LMG 26379T, Bosea lupini LMG 26383T, Bosea eneae CCUG 43111T, Bosea vestrisii CCUG 43114T and Bosea massiliensis CCUG 43117T of 99.8, 99.1, 98.4, 98.4, 98.4 and 98.2 %, respectively. The average nucleotide identity value between strain 1131T and Bosea vaviloviae Vaf-18T was 93.4 % and the DNA relatedness was 38 %. The primary cellular fatty acids of strain 1131T were C16 : 1ω7c and C18 : 1ω7c. The primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major compound in the quinone system was ubiquinone Q-10 and in the polyamine pattern sym-homospermidine was predominant. Additional phenotypic characteristics included growth at 5–35 °C, pH values of pH 5.5–8.0, a salt tolerance range of 0.0–1.2 % (w/v), and production of an unknown water soluble brown pigment. After phenotypic, chemotaxonomic and genomic analyses, this isolate was identified as a novel species for which the name Bosea psychrotolerans is proposed. The type strain is 1131T (NRRL B-65405=LMG 30034).

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