Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys

Abstract

Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys Two facultatively anaerobic, budding bacterial strains, designated W1215-PCA4 T and SRNK-1, were isolated from water from Lake Michigan, USA. The two strains showed identical ERIC-PCRgenerated genomic fingerprints and shared 99.9 % 16S rRNA gene sequence similarity. Strain W1215-PCA4 T showed highest 16S rRNA gene sequence similarities to Labrys monachus VKM B-1479 T (95.8 %), Labrys methylaminiphilus DSM 16812 T (95.1 %), Labrys okinawensis MAFF 210191 T (96.0 %), Labrys miyagiensis G24103 T (95.4 %), Labrys neptuniae BCRC 17578 T (95.7 %) and Labrys portucalensis DSM 17916 T (95.8 %). Data suggested that the two strains were members of a single novel species of the genus Labrys. The major cellular fatty acids of the two isolates were C 18 : 1 v7c, C 19 : 0 cyclo v8c and C 16 : 0 . Their polar lipid profiles were highly similar to that of Labrys monachus DSM 5896 T . The primary quinone was ubiquinone Q-10, with minor amounts of Q-9 and Q-11. sym-Homospermidine was the predominant polyamine, with putrescine present in moderate amounts. The two strains were identical in terms of their biochemical and physiological traits, but were distinguishable from other species of the genus Labrys. Hence, the description of a novel species in this genus appears to be justified. The name Labrys wisconsinensis sp. nov. is proposed; the type strain is W1215-PCA4 T (5DSM 19619 T 5NRRL B-51088 T ).

Labrys soli sp. nov., isolated from the rhizosphere of ginseng

“…The major fatty acids and quinone of DCY64 T are in good agreement with those of member of the genus Labrys (Islam et al , 2007). The polar lipids patterns of DCY64 T and three reference type strains in this study are consistent with those described as part of a previous study by Albert et al (2010). Major amounts of phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol, minor to trace amounts of phosphatidyldethanolamine, phosphatidyldimethylethanolamine, two unidentified aminolipids, three unidentified aminophospholipids, four unidentified phospholipids and two unidentified polar lipids were detected in the polar lipids pattern of strain DCY64 T (Fig.…”

“…The three phylogenetic analysis methods () clearly showed that DCY64 T clustered within the group containing members of the genus Labrys . This cluster was supported by a 100 % bootstrap value that is consistent with the results of previous studies (Miller et al , 2005; Islam et al , 2007; Chou et al , 2007; Carvalho et al , 2008; Albert et al , 2010). However, DCY64 T formed a monophyletic branch in this group.…”

“…Islam et al (2007) emended the genus with information on cell morphology, motility, DNA G+C content (range of 61.0–66.0 mol%), major fatty acids (C 19 : 0 cyclo ω8 c , C 16 : 0 , C 18 : 0 and C 18 : 1 ω7 c ) and quinone (Q-10). Subsequently, this genus was further emended by Albert et al (2010), including that members of the genus are aerobes or facultative anaerobes; diphosphatidylglycerol, phosphatidylmonomethylthanolamine and phosphatidylcholine are major polar lipids; and sym -homospermidine is the major polyamine. At the time of writing, the genus Labrys includes seven species with validly published names isolated from different environments, such as Labrys monachus isolated from silt of a lake (Vasilyeva & Semenov, 1984), Labrys methylaminiphilus isolated from a freshwater sediment (Miller et al , 2005), Labrys okinawensis and Labrys miyagiensis isolated from rhizosphere (Islam et al , 2007), Labrys neptuniae isolated from root nodules (Chou et al , 2007), Labrys portucalensis isolated from sediment (Carvalho et al , 2008) and Labrys wisconsinensis isolated from water (Albert et al 2010).…”

“…This profile slightly differed with respect to those of reference type strains in this study by the absence of C 16 : 0 2-OH, C 16 : 0 3-OH and C 18 : 1 2-OH. The differences in relative amounts of summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and C 19 : 1 cyclo ω8 c between strain DCY64 T and reference type strains were not considered to be of relevance for discrimination between species as described by Albert et al (2010). The quinone systems contained predominantly ubiquinone Q-10 (98.5 %) and trace amounts of Q-11 (1.5 %).…”

Filimonas aurantiibacter sp. nov., an orange-pigmented bacterium isolated from lake water and emended description of the genus Filimonas

“…They reported that Betaproteobacteria predominated, followed by Bacteroidetes and Alphaproteobacteria. There are however, no published reports that characterized the microbial community in Lake Michigan potable water, although Albert et al (2010Albert et al ( , 2013Albert et al ( , 2014 have reported the isolation of several novel bacterial species and a novel genus of bacteria from Lake Michigan potable water.…”