Dihydrodipicolinate synthase (DHDPS) is the main enzyme of a specific branch of the aspartate pathway leading to lysine biosynthesis in higher plants. We have cloned and characterized the DHDPS gene from Zizania latifolia Griseb, which was named ZlDHDPS. Sequence analysis indicates that it contains an ORF of 954 bp interrupted by two exons and one intron encoding a polypeptide of 317 amino acids lacking a plastid transit peptide and a stop codon. The sequence of ZlDHDPS has high identity with known plant DHDPS in GenBank. Southern blotting analysis indicates that there are two copies of Z. latifolia DHDPS (ZlDHDPS) gene in the Z. latifolia nuclear genome. RT-PCR analysis shows the expression of ZlDHDPS is tissue specific and high level expression is present in fast-growing tissue and reproductive tissue. The 5′-regulatory sequence of ZlDHDPS contains a GT-1 box and a (CA)n element, which may play a role in regulating the expression of ZlDHDPS. The fusion construct of the ZlDHDPS sequence with the reporter gene GUS was transiently expressed in the onion epidermal cells by particle gun-mediated bombardment suggesting that ZlDHDPS was located in the cytoplasm, different from DHDPS gene of other species. Functional complementary analysis showed that ZlDHDPS can recover the DHDPS-deleted mutant of Escherichia coli.

No abstract

Soil organic cover technology is an effective way to solve agricultural productivity and agro-environmental issues. However, some factors limit the widespread adoption of its application, including unclear soil organic cover effects and the lack of high-performance soil organic cover machinery. Aiming to solve the existing problems, improve soil organic cover quality, and reduce energy consumption, this article reviewed the common and commercial machines by reviewing the existing literature and company products. The current problems and technical difficulties of the machines were expounded. Simultaneously, the method of design and optimization of the device (chopper, spreader, rotary tiller, and plow) and its key parts (blades, discs, and plows) were reviewed. Furthermore, the features and differences of the devices and their key parts were compared, and their advantages and disadvantages were analyzed. In conclusion, the future directions for soil organic cover technology and machinery development were suggested, including clarifying the soil organic cover effect, selecting the suitable soil organic cover patterns, and developing soil organic cover machinery.