Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance

Shen, Weiwei; Song, Chengli; Chen, Jie; Fu, Yaping; Wu, Jianli; Jiang, Shaomei

doi:10.1016/s1672-6308(11)60003-6

Cited by 6 publications

(8 citation statements)

References 21 publications

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“…Agronomically, relative to Oryza, Z. latifolia generally has higher resistance to abiotic (such as cold) and biotic stresses (such as rice blast) Shen et al, 2011), and higher biomass production. Therefore, the Zizania genomes provide a genetic resource for rice breeding.…”

Section: Discussionmentioning

confidence: 99%

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A host plant genome (Zizania latifolia) after a century‐long endophyte infection

et al. 2015

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These authors contributed equally to this work. SUMMARYDespite the importance of host-microbe interactions in natural ecosystems, agriculture and medicine, the impact of long-term (especially decades or longer) microbial colonization on the dynamics of host genomes is not well understood. The vegetable crop 'Jiaobai' with enlarged edible stems was domesticated from wild Zizania latifolia (Oryzeae) approximately 2000 years ago as a result of persistent infection by a fungal endophyte, Ustilago esculenta. Asexual propagation via infected rhizomes is the only means of Jiaobai production, and the Z. latifolia-endophyte complex has been maintained continuously for two centuries. Here, genomic analysis revealed that cultivated Z. latifolia has a significantly smaller repertoire of immune receptors compared with wild Z. latifolia. There are widespread gene losses/mutations and expression changes in the plant-pathogen interaction pathway in Jiaobai. These results show that continuous long-standing endophyte association can have a major effect on the evolution of the structural and transcriptomic components of the host genome.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, the Zizania genomes provide a genetic resource for rice breeding. Efforts to transfer the Zizania genetic background into Oryza via distant hybridization have been successfully attempted over the last 15 years (Liu et al, 1999;Shen et al, 2011). The Zizania genome will also provide a diverse gene pool for rice molecular breeding.…”

Section: Discussionmentioning

confidence: 99%

A host plant genome (Zizania latifolia) after a century‐long endophyte infection

et al. 2015

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“…China (Liu et al, 1999(Liu et al, , 2007Yu et al, 2006;Zhou et al, 2007;Shen et al, 2011;Peng et al, 2013;Wang et al, 2013). Natural populations of Z. latifolia are distributed in the East of China along a wide stretch of latitudinal zones (21 • -50 • N).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of wild rice Zizania latifolia in an expansive heterogeneous landscape along a latitudinal gradient

Wagutu

Fan

et al. 2022

Front. Ecol. Evol.

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Global aquatic habitats are undergoing rapid degradation and fragmentation as a result of climate change and changes in land use. Understanding the genetic variability and adaptive potential of aquatic plant species is thus important for conservation purposes. In this study, we investigated the genetic diversity and structure of the extant natural populations of Zizania latifolia from five river basins in China based on 46 microsatellite markers. We tested isolation by environment (IBE), isolation by resistance (IBR), and isolation by distance (IBD) patterns using a reciprocal causal model (RCM). Furthermore, we elucidated the impact of the environment on Z. latifolia genetic diversity using generalized linear models (GLMs) and spatially explicit mixed models. Low genetic diversity (HE = 0.125–0.433) and high genetic differentiation (FST = 0.641, Øpt = 0.654) were found. Higher historical gene flow (MH = 0.212–2.354) than contemporary gene flow (MC = 0.0112–0.0247) and significant bottlenecks in almost all populations were identified, highlighting the negative impact of wetland fragmentation. The IBE model was exclusively supported for all populations and in three river basins. The IBD and IBR models were supported in one river basin each. The maximum temperature of the warmest month and precipitation seasonality were the plausible environmental parameters responsible for the observed pattern of genetic diversity. Local adaptation signatures were found, with nine loci identified as outliers, four of which were gene-linked and associated with environmental variables. Based on these findings, IBE is more important than IBD and IBR in shaping the genetic structure of Z. latifolia.

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“…To analyze the possible involvement of OsASR6 in rice defense, we examined its expression in response to bacterial pathogens Xoc strain oxy04 and Xoo strain PXO99 in wild-type (WT) rice cultivar Nipponbare, which carries no known R gene conferring resistance to the above strains and thus is susceptible to them [ 14 ]. OsASR6 expression in oxy04-inoculated rice leaves was significantly induced by 19.2 folds, compared with that in the sterilized H 2 O mock-infiltrated leaves at 5 d post inoculation (dpi) when the Xoc -infiltrated areas just began to show water-soaked symptoms.…”

Section: Resultsmentioning

confidence: 99%

OsASR6 Alleviates Rice Resistance to Xanthomonas oryzae via Transcriptional Suppression of OsCIPK15

Guo

Chen

et al. 2022

IJMS

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The plant-specific ASR (abscisic acid, stress and ripening) transcription factors are pivotal regulators of plant responses to abiotic stresses. However, their functions in plant disease resistance remain largely unknown. In this study, we revealed the role of OsASR6 in rice plants’ resistance to two important bacterial diseases caused by Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) and elucidated the mechanisms underlying OsASR6-regulated resistance. The expression of OsASR6 was strongly elevated in response to both Xoo and Xoc challenges. Silencing of OsASR6 in OsASR6-RNAi transgenic plants markedly enhanced rice resistance to the two bacterial pathogens. Moreover, comparative transcriptome analyses for OsASR6-RNAi and wild-type plants inoculated and uninoculated with Xoc demonstrated that OsASR6 suppressed rice resistance to Xoc by comprehensively fine-tuning CIPK15- and WRKY45-1-mediated immunity, SA signaling and redox homeostasis. Further luciferase reporter assays confirmed that OsASR6 negatively regulated CIPK15 but not WRKY45-1 expression in planta. Overexpression of OsCIPK15 strongly enhanced rice resistance to Xoo and Xoc. Collectively, these results reveal that OsASR6 alleviates rice resistance through the transcriptional suppression of OsCIPK15, and thus links calcium signaling to rice resistance against X. oryzae. Our findings provide insight into the mechanisms underlying OsASR6-mediated regulation of rice resistance to X. oryzae.

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Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance

Cited by 6 publications

References 21 publications

A host plant genome (Zizania latifolia) after a century‐long endophyte infection

A host plant genome (Zizania latifolia) after a century‐long endophyte infection

Genetic structure of wild rice Zizania latifolia in an expansive heterogeneous landscape along a latitudinal gradient

OsASR6 Alleviates Rice Resistance to Xanthomonas oryzae via Transcriptional Suppression of OsCIPK15

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