OsASR6 Alleviates Rice Resistance to Xanthomonas oryzae via Transcriptional Suppression of OsCIPK15

Guo, Wei; Chen, Songyu; Xu, You‐Ping; Cai, Xin-Zhong

doi:10.3390/ijms23126622

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…In addition to singleton DR genes, some DR gene families, such as OsWRKY45-1 and OsWRKY45-2 [ 12 ], the polygalacturonase inhibiting protein genes OsPGIP1 and OsPGIP4 [ 13 ], and the salicylic acid (SA) metabolic enzyme family OsF3H03g , OsF3H04g , and OsS3H [ 14 – 16 ], have also been reported to be involved in BLS resistance. Some transcription modules, such as OsHsfB4d-OsHsp18.0-CI [ 17 ] and OsASR6-OsCIPK15 [ 18 ], are also involved in BLS resistance. Nonetheless, the defense mechanisms against BLS, particularly the intricacies of the signaling networks associated with these DR genes, remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Bi,

Yu,

Mao

et al. 2024

BMC Plant Biol

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Background Two-tiered plant immune responses involve cross-talk among defense-responsive (DR) genes involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), effector-triggered immunity (ETI) and effector-triggered susceptibility (ETS). Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an important bacterial disease that causes serious threats to rice yield and quality. Transcriptomic profiling provides an effective approach for the comprehensive and large-scale detection of DR genes that participate in the interactions between rice and Xoc. Results In this study, we used RNA-seq to analyze the differentially expressed genes (DEGs) in susceptible rice after inoculation with two naturally pathogenic Xoc strains, a hypervirulent strain, HGA4, and a relatively hypovirulent strain, RS105. First, bacterial growth curve and biomass quantification revealed that differential growth occurred beginning at 1 day post inoculation (dpi) and became more significant at 3 dpi. Additionally, we analyzed the DEGs at 12 h and 3 days post inoculation with two strains, representing the DR genes involved in the PTI and ETI/ETS responses, respectively. Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed on the common DEGs, which included 4380 upregulated and 4019 downregulated genes and 930 upregulated and 1383 downregulated genes identified for the two strains at 12 h post inoculation (hpi) and 3 dpi, respectively. Compared to those at 12 hpi, at 3 dpi the number of common DEGs decreased, while the degree of differential expression was intensified. In addition, more disease-related GO pathways were enriched, and more transcription activator-like effector (TALE) putative target genes were upregulated in plants inoculated with HGA4 than in those inoculated with RS105 at 3 dpi. Then, four DRs were randomly selected for the BLS resistance assay. We found that CDP3.10, LOC_Os11g03820, and OsDSR2 positively regulated rice resistance to Xoc, while OsSPX3 negatively regulated rice resistance. Conclusions By using an enrichment method for RNA-seq, we identified a group of DEGs related to the two stages of response to the Xoc strain, which included four functionally identified DR genes.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Bi,

Yu,

Mao

et al. 2024

BMC Plant Biol

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“…Mainly, the quantitative trait loci (QTLs) are responsible for rice BLS resistance [42][43][44][45]. Moreover, the constitutive expression of OsMPK6 [46], OsGH3-2 [47], OsPGIP4 [48], OsHSP18.0-CI [49], OsMAPK10.2 [50], OsPSKR1 [51], OsPGIP1 [52], OsHSFB4d [53], OsPDX1.2 [54], and OsCIPK15 [55] enhances rice resistance to BLS. The loss or depression of the function of OsNRRB [56], OsImpα1a and OsImpα1b [57], OsALDH2B1 [58], OsEDR1 [59], OsAPS1 [60], OsF3H03g [28], and OsNRAMP1 [61] increases BLS resistance in rice.…”

Section: Introductionmentioning

confidence: 99%

Activated Expression of Rice DMR6-like Gene OsS3H Partially Explores the Susceptibility to Bacterial Leaf Streak Mediated by Knock-Out OsF3H04g

Wu,

Bi,

et al. 2023

IJMS

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Downy Mildew Resistance 6-like (DMR6-like) genes are identified as salicylic acid (SA) hydroxylases and negative regulators of plant immunity. Previously, we identified two rice DMR6-like genes, OsF3H03g, and OsF3H04g, that act as susceptible targets of transcription activator-like effectors (TALEs) from Xanthomonas oryzae pv. oryzicola (Xoc), which causes bacterial leaf streak (BLS) in rice. Furthermore, all four homologs of rice DMR6-like proteins were identified to predominantly carry the enzyme activity of SA 5-hydroxylase (S5H), negatively regulate rice broad-spectrum resistance, and cause the loss of function of these OsDMR6s, leading to increased resistance to rice blast and bacterial blight (BB). Here, we curiously found that an OsF3H04g knock-out mutant created by T-DNA insertion, osf3h04g, was remarkedly susceptible to BLS and BB and showed an extreme reduction in SA content. OsF3H04g knock-out rice lines produced by gene-editing were mildly susceptible to BLS and reduced content of SA. To explore the susceptibility mechanism in OsF3H04g loss-of-function rice lines, transcriptome sequencing revealed that another homolog, OsS3H, had induced expression in the loss-of-function OsF3H04g rice lines. Furthermore, we confirmed that a great induction of OsS3H downstream and genomically adjacent to OsF3H04g in osf3h04g was primarily related to the inserted T-DNA carrying quadruple enhancer elements of 35S, while a slight induction was caused by an unknown mechanism in gene-editing lines. Then, we found that the overexpression of OsS3H increased rice susceptibility to BLS, while gene-editing mediated the loss-of-function OsS3H enhanced rice resistance to BLS. However, the knock-out of both OsF3H04g and OsS3H by gene-editing only neutralized rice resistance to BLS. Thus, we concluded that the knock-out of OsF3H04g activated the expression of the OsS3H, partially participating in the susceptibility to BLS in rice.

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OsBLS6.2: A rice bacterial leaf streak resistance gene identified by GWAS and RNA-seq

Xie,

Lin,

et al. 2023

The Crop Journal

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OsASR6 Alleviates Rice Resistance to Xanthomonas oryzae via Transcriptional Suppression of OsCIPK15

Cited by 6 publications

References 33 publications

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Activated Expression of Rice DMR6-like Gene OsS3H Partially Explores the Susceptibility to Bacterial Leaf Streak Mediated by Knock-Out OsF3H04g

OsBLS6.2: A rice bacterial leaf streak resistance gene identified by GWAS and RNA-seq

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