Melanoma is the foremost malignant cutaneous cancer and it is extremely resistant to chemotherapy and radiotherapy. Curcumin is an active component of turmeric, the yellow spice derived from the rhizome of Curcuma longa, and is widely known for its anti-inflammatory and anti-cancerogenic properties. Several recent studies suggest that curcumin induces apoptosis by modulating multiple signaling pathways to exert its anticancer effect. In the present study, we investigated the effect of curcumin on the viability, invasion potential, cell cycle, autophagy and the AKT, mTOR, P70S6K proteins of AKT/mTOR signaling pathway in human melanoma A375 and C8161 cell lines in vitro and in an in vivo tumorigenesis model. Curcumin effectively inhibited the proliferation of melanoma cells in vitro and in vivo. It suppressed cell invasion, arrested the cancer cells at G2/M phase of the cell cycle, and induced autophagy. Furthermore, curcumin suppressed the activation of AKT, mTOR and P70S6K proteins. Curcumin, therefore, is a potent suppressor of cell viability and invasion, and simultaneously an inducer of autophagy in A375 and C8161 cells. Accordingly, curcumin could be a novel therapeutic candidate for the management of melanoma.

No abstract

Abstract-In this paper, a miniaturized printed dipole antenna with the V-shaped ground is proposed for radio frequency identification (RFID) readers operating at the frequency of 2.45 GHz. The principles of the microstrip balun and the printed dipole are analyzed and design considerations are formulated. Through extending and shaping the ground to reduce the coupling between the balun and the dipole, the antenna's impedance bandwidth is broadened and the antenna's radiation pattern is improved. The 3D finite difference time domain (FDTD) Electromagnetic simulations are carried out to evaluate the antenna's performance. The effects of the extending angle and the position of the ground are investigated to obtain the optimized parameters. The antenna was fabricated and measured in a microwave anechoic chamber. The results show that the proposed antenna achieves a broader impedance bandwidth, a higher forward radiation gain and a stronger suppression to backward radiation compared with the one without such a ground.

Abstract-The ambiguity functions of a kind of direct chaotic radar system are investigated. In this radar system, a microwave chaotic Colpitts oscillator is employed to generate the source signal that is directly transmitted through a wideband antenna without modulation. The auto-ambiguity function of this radar system shows many sidelobes which makes the unambiguous detection difficult. It is because the spectrum of the chaotic signal is not very flat and smooth, with pulsation peaks in it. The cross-ambiguity functions of the direct radar system have also been investigated to evaluate the electronic counter countermeasure (ECCM) performance and the "multi-user" characteristic. It is shown that rather excellent ECCM capability can be achieved in this radar system with transmitting chaotic signals generated by circuits with same parameters but at different time or with slightly different circuit parameters. In addition, several possible methods to reshape the spectrum of the chaotic signal from microwave Colpitts oscillators to improve the unambiguous detection performance are suggested at the end of this paper.

Anti-GABA receptor encephalitis is a rare, unique autoimmune disease, and is often associated with tumors. It should be considered in the differential diagnosis for middle and senior-aged patients who present with predominantly limbic encephalitis symptoms. Importantly, earlier recognition of this potentially treatable condition could improve its overall prognosis.

Background: The pathological hallmarks of Alzheimer's disease (AD) involve alterations in the expression of numerous genes associated with transcriptional levels, which are determined by chromatin accessibility. Here, the landscape of chromatin accessibility was studied to understand the outline of the transcription and expression of AD-associated metabolism genes in an AD mouse model. Methods: The assay for transposase-accessible chromatin by sequencing (ATAC-seq) was used to investigate the ADassociated chromatin reshaping in the APPswe/PS1dE9 (APP/PS1) mouse model. ATAC-seq data in the hippocampus of 8-month-old APP/PS1 mice were generated, and the relationship between chromatin accessibility and gene expression was analyzed in combination with RNA sequencing. Gene ontology (GO) analysis was applied to elucidate biological processes and signaling pathways altered in APP/PS1 mice. Critical transcription factors were identified; alterations in chromatin accessibility were further confirmed using chromatin immunoprecipitation assays. Results: We identified 1690 increased AD-associated chromatin-accessible regions in the hippocampal tissues of APP/ PS1 mice. These regions were enriched in genes related to diverse signaling pathways, including the PI3K-Akt, Hippo, TGF-β, and Jak-Stat signaling pathways, which play essential roles in regulating cell proliferation, apoptosis, and inflammatory responses. A total of 1003 decreased chromatin-accessible regions were considered to be related with declined AD-associated biological processes including cellular response to hyperoxia and insulin stimulus, synaptic transmission, and positive regulation of autophagy. In the APP/PS1 hippocampus, 1090 genes were found to be upregulated and 1081 downregulated. Interestingly, enhanced ATAC-seq signal was found in approximately 740 genes, with 43 exhibiting upregulated mRNA levels. Several genes involved in AD development were found to have a significantly increased expression in APP/PS1 mice compared to controls, including Sele, Clec7a, Cst7, and Ccr6. The signatures of numerous transcription factors, including Olig2, NeuroD1, TCF4, and NeuroG2, were found enriched in the AD-associated accessible chromatin regions. The transcription-activating marks of H3K4me3 and H3K27ac were also found increased in the promoters of these genes. These results indicate that the mechanism for the upregulation of genes could be attributed to the enrichment of open chromatin regions with transcription factors motifs and the histone marks H3K4me3 and H3K27ac.

With the development of 3D scanning technology, a huge volume of point cloud data has been collected at a lower cost. The huge data set is the main burden during the data processing of point clouds, so point cloud simplification is critical. The main aim of point cloud simplification is to reduce data volume while preserving the data features. Therefore, this paper provides a new method for point cloud simplification, named FPPS (feature-preserved point cloud simplification). In FPPS, point cloud simplification entropy is defined, which quantifies features hidden in point clouds. According to simplification entropy, the key points including the majority of the geometric features are selected. Then, based on the natural quadric shape, we introduce a point cloud matching model (PCMM), by which the simplification rules are set. Additionally, the similarity between PCMM and the neighbors of the key points is measured by the shape operator. This represents the criteria for the adaptive simplification parameters in FPPS. Finally, the experiment verifies the feasibility of FPPS and compares FPPS with other four-point cloud simplification algorithms. The results show that FPPS is superior to other simplification algorithms. In addition, FPPS can partially recognize noise.

POLD1, the catalytic subunit of DNA Pol δ, plays an important role in DNA synthesis and DNA damage repair, and POLD1 is downregulated in replicative senescence and mediates cell aging. However, the mechanisms of age-related downregulation of POLD1 expression have not been elucidated. In this study, four potential CpG islands in the POLD1 promoter were found, and the methylation levels of the POLD1 promoter were increased in aging 2BS cells, WI-38 cells and peripheral blood lymphocytes, especially at a single site, CpG 36, in CpG island 3. Then, the transcription factor E2F1 was observed to bind to these sites. The binding affinity of E2F1 for the POLD1 promoter was found to show age-related attenuation and was confirmed to be positively regulated by the E2F1 level and negatively regulated by POLD1 promoter methylation. Moreover, cell senescence characteristics were observed in the cells transfected with shRNA-E2F1 and could contribute to the downregulation of POLD1 induced by the E2F1 decline. Collectively, these results indicated that the attenuation of the binding affinity of E2F1 for the POLD1 promoter, mediated by an age-related decline in E2F1 and increased methylation of CpG island 3, downregulates POLD1 expression in aging.