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The aim of this study was to use IEDB software to predict the suitable MERS-CoV epitope vaccine against the most known world population alleles through four selecting proteins such as S glycoprotein and envelope protein and their modification sequences after the pandemic spread of MERS-CoV in 2012. IEDB services is one of the computational methods; the output of this study showed that S glycoprotein, envelope (E) protein, and S and E protein modified sequences of MERS-CoV might be considered as a protective immunogenic with high conservancy because they can elect both neutralizing antibodies and T-cell responses when reacting with B-cell, T-helper cell, and cytotoxic T lymphocyte. NetCTL, NetChop, and MHC-NP were used to confirm our results. Population coverage analysis showed that the putative helper T-cell epitopes and CTL epitopes could cover most of the world population in more than 60 geographical regions. According to AllerHunter results, all those selected different protein showed non-allergen; this finding makes this computational vaccine study more desirable for vaccine synthesis.

Back ground: Cytomeglovirus(CMV) is a common viral infection globally. Although most infections are subclinical; infection during pregnancy may be associated with serious outcome such as abortion, stillbirth and congenitally malformed child. Methods and results: During the period from June to July2003, 250 subjects (150 blood donors and 100 women presenting to Khartoum Teaching Hospital's and Omdurman Maternity Hospital respectively)had their blood tested for IgG antibodies against Cytomegalovirus using ELISA test. Of these 84% were found to harbor Cytomegalovirus antibodies. The seroprevalence of the virus was higher in the antenatal women (95%) compared to the blood donors (77%). Conclusion: The study revealed insignificant association between infection with the virus and both history of previous surgical operation and blood transfusion (p> 0.05).CMV infection was found to be associated with increased risk of abortion in women under study.

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