2The concept of DNA based stabilization of nanostructures to enhance the surface 3 reactivity has been focus of great interest in the design of colorimetric aptaswitches.
4Whereas, colorimetric methodologies have limited sensitivity, this concept is rarely 5 considered for other sensing approaches such as those based on the fluorescence detection. In 6 this paper, we have investigated the impact of reversible assembly of single strand DNA 7 aptamer on nanoparticles surface chemistry, involving target tuneable electrostatic and steric 8 repulsion phenomena for fluorescence based detection of molecular interaction. In the same 9 context, literature reported fluorescence based aptamer assays are prone to certain limitations 10 such as complicated labelling chemistry, less conjugation yield, low binding affinity and 11 elevated cost per assay. Alternatively, our designed aptaswitch capitalizes on the surface 12 chemistry of nanoparticles to quench the response of fluorescence particles, eliminating the 13 need of bioconjugation with fluorophore. As a proof of concept, the proposed methodology 14 was used for the detection of ochratoxin A with TiO 2 nanoparticles as representative 15 nanomaterial. We expect that this concept may pave a new way to probe aptamer-target 16 binding event, since any nanomaterials with fluorescence quenching characteristics can be 17 regulated in the same manner.18 19
Gratiola officinalis Linn. (Plantaginaceae), commonly known as common Hedgehyssop or "Herb of Grace" is well-known for its pharmacological properties. Various parts of this plant (root and herb) are being used in phytomedicinnes. This review focuses on the classification, etymology, habitat and distribution, botanical description, medicinal, food and miscellaneous uses and structures of various bioactive constituents of its multipurpose fruit. Being a rich source of bioactive constituents of therapeutic value, G. officinalis is used as a potent medicine to treat gout, mild dropsy, sciatica, external ulcers, intestinal worms, chronic eczema, and persistent itching skin.
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