This study includes (100) samples which grouped as following: control group ( 35) sample (blood and benign), patients group including (30) blood samples and (35) FFPE samples (tissue imbed in paraffin).The age of study samples were ranged from 30-89 years old men and women). These samples were grouped in six aging groups that each 10 years in one group. results indicate that the age risk of disease incidence is begin in 50 years old and increased between 60-69 years old for both sexes.The CDK1 gene was detected by PCR-sequencing in order to investigate if there were any genetic defect within this gene. The alignment results of the 254 bp samples revealed the presence ofSNP (rs2456777) that distributed variably in the analyzed samples in comparison with the referring reference DNA sequences. The results showed that the allele G is responsible for the appearance of disease and its progression in patientsby reaching to metastasis stage and have gene expression by tumor molecular phenotype, with significant value in tissue samples.
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