Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium. Biofuel Research Journal 10 (2016) HIGHLIGHTSUsing different media for inoculums preparation and microalgae production improved lipid and biomass productivities.When SH4 was selected for inoculation medium and N8 was selected for production medium, 130% increase in biomass productivity and 40% increase in lipid productivity was observed. Specific growth rate improved by differing inoculums preparation medium from production medium and changed from 0.0040/h to 0.0122/h. Improvement of biomass and lipid productivities is now one of the main concerns in commercialization of microalgae cultivation as a feedstock for algal biofuel production. Conventional photoautotrophic processes using well-studied and rich in oil strain of Chlorella vulgaris are not able to meet such demands. A new strategy of inoculating algae production medium with cells grown in a different medium from the production medium was proposed herein. More specifically, when SH4 was used as production medium and N8 was used as inoculation medium, biomass and lipid productivities increased by 2.33 folds and 1.44 folds, respectively, compared with when the production and inoculation media were the same, such as SH4. The findings of the present investigation showed that this cultivation scheme resulted in 52% increase in cell number and 54% increase in dry weight leading to improved productivities. Although by even considering this improvement, photoautotrophic cultivation of algae can hardly compete with the heterotrophic cultivation, the high cost of hydrocarbon supply required in large-scale heterotrophic processes marks the technique proposed in the present study as a promising approach for commercialization of algal biofuel production. ARTICLE INFO ABSTRACT © 2016 BRTeam. All rights reserved.Journal homepage: www.biofueljournal.com Hamedi et al. / Biofuel Research Journal 10 (2016) 410-416 Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium.
The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.
Microalgae are known for lipid production as source of biofuels. Chlorella vulgaris was studied in terms of biomass production and lipid content in N8 and SH4 media inoculated with cells grown in each of the two media. Observations revealed that for biomass production N8 medium inoculated with cells grown in SH4 medium is preferred. For lipid production N8 medium is also preferred, inoculated with cells grown in N8 medium.
The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study for the first time, the spouted bed reactors were utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at the T= 50 °C and Q= 1.4. In order to investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that optimum pH was decreased about 12.3% while 85.7% increase was observed in optimum temperature of DEE compare to FEE. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.
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