A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Abstract

The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study for the first time, the spouted bed reactors were utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at the T= 50 °C and Q= 1.4. In order to investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that optimum pH was decreased about 12.3% while 85.7% increase was observed in optimum temperature of DEE compare to FEE. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.