A total of 69 isolates of Pseudomonas aeruginosa were obtained from different clinical samples including wounds 24 (35%) and burns 67 (65%)، the genes that responsible for Pseudomonas aeruginosa resistance isolates was detected, the results showed that quaternary ammonium compound delta 1 (qacE∆1) gene was 97.1% in Pseudomonas aeruginosa isolation. 24 (35%) in the wounds and 63 (62.31%) in burns, the size of the gene showed 285 bp, the results of the qacE∆1 analysis showed a single mutation of the mutation type. The results of genetic analysis tree were showed after comparing presence of genetic variations of the qacE∆1 gene with their relatives from the nucleotide sequences as containing variations occurring at one distance and equally in Pseudomonas aeruginosa and other bacterial species.
From 50 stool samples collected from children with diarrhea of both sexes who visited various hospitals in Baghdad, 26 isolates of E.coli were found to belong to the phylogenetic group E. The findings revealed that the percentage of E.coli for thephylogenetic group E is (52%) , making it the dominant group among the other phylogenetic groups. The findings demonstrated that 100% of the E.coli isolates from phylogenetic group E are resistant to penicillin, and only 15% are resistant to imipenem. Multi-drug resistance (MDR) was found to be 15%, while XDR reached 85%. The results of thephylogenetic group for the remaining species of isolates in this study were group A (2/50 and by 4%), group B2 (1/50 and by 2% ),group C (12/50 and by 24%), group D (6/50 and by 12%), group F (3/50 and by 6%), group B1 by 0%, and group Clade 1 by (0%).
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