DNA Sequences of qacE∆1 Gene in Pseudomonas Aeruginosa Isolated from Wounds and Burns Infections.

Al-Azzawi, Shahla Najim Abed; Abdullah, Rana Mujahid

doi:10.32894/kujss.2019.14.3.2

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…Conversely, the IMVIC test suite, encompassing Indole production, Methyl Red (MR), Vocus-Proscar (VP), and Citrate utilization examinations, yielded predominantly negative results, except for the Citrate utilization test which yielded affirmative results. Notably, these findings concur with investigations conducted within Iraq (13) The current investigation documented the percentage of resistance displayed by P. aeruginosa bacteria towards Fluoroquinolones, a class of antibiotics renowned for their efficacy against this bacterial strain. The results indicated the highest resistance of Levofloxacin (LEV) and Ciprofloxacin (CIP) (38% and 22%, respectively).…”

Section: Discussionsupporting

confidence: 89%

Molecular Identification of lasB gene in Pseudomonas Aeruginosa Isolated From Patients With Urinary Tract Infection

Jumaah alhussona,

Hussein Jalood

2024

JEPS

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Background: Pseudomonas aeruginosa is an opportunistic pathogen capable of infecting various body sites due to the presence of multiple virulence factors that significantly contribute to disease. This study aimed to assess the antibiotic susceptibility of P. aeruginosa isolates, detect the frequency of the lasB gene in clinical isolates, furthermore analyze the genetic sequence and the resulting mutations.Materials and Methods: A total of 120 samples were collected from patients with urinary tract infections (UTIs) during the period from November 2021 to March 2022. The specimens identified using microbiological and biochemical tests, as well as the VITEK2 system, and confirmed by PCR using specific primers. Gene sequence analysis was conducted by aligning the sequences with those in the gene bank using the Blast alignment program. Antibiotic sensitivity testing of Pseudomonas aeruginosaisolates was performed using the Kirby-Bauer method.Results: Out of the 120 samples, 18 were positive for P. aeruginosa isolates. The infection rate was higher in females (88.8%) compared to males (11.2%). The highest resistance rate was observed for Levofloxacin (38%), while Piperacillin/Tazobactam and Meropenem showed the lowest resistance rates (11% each). The lasB gene was detected in all 18 isolates, and gene sequence analysis confirmed their identity as P. aeruginosa, matching the reference sequence in the gene bank. The gene sequence analysis of the lasB gene revealed the presence of five mutations, Conclusions: This study demonstrated that Pseudomonas aeruginosa possesses several virulence factors, including the lasB gene. Sequencing analysis of the lasB gene revealed the presence of multiple mutations.

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Section: Discussionsupporting

confidence: 89%

Molecular Identification of lasB gene in Pseudomonas Aeruginosa Isolated From Patients With Urinary Tract Infection

Jumaah alhussona,

Hussein Jalood

2024

JEPS

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“…The polymerase chain reaction used for the detection of 25 DNA samples of carbapenem resistance P. aeruginosa isolates, the results of the reaction and gel electrophoresis revealed the alg D gene percentage was 100% with bands appearing within the estimated size of the gene (1310 bp.) that showed in the Figure (4). This result is consistent with Ellappan et al 2018 who indicated that alg D was 93% of carbapenem resistance isolates they indicated that the coexistence of different antimicrobial resistance mechanisms together with virulence genes in carbapenem-resistant isolates has become an alarming increasing threat, constantly monitoring of multidrug-resistant pathogens is critical for clinicians to identify treatment options against such infections (Ellappan et al, 2018).…”

Section: Resultssupporting

confidence: 82%

Carbapenem Resistance Related with Biofilm Formation and Pilin Genes in Clinical Pseudomonas aeruginosa Isolates

Tiba A. Al-Mohammed,

Huda. M. Mahmood

2024

IJPS

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Pseudomonas aeruginosa is a common cause of nosocomial infections worldwide, and infections caused by this bacterial organism are difficult to eradicate because it is intrinsically resistant or less susceptible to several antimicrobial agents. The study aimed to identify the genotype distribution or frequency of virulence factors genes (algD, pilA, and pilB) involved in alginate and the type 3 system of carbapenem-resistant of P. aeruginosa local isolates. The genotype distribution of 25 carbapenem-resistant P. aeruginosa involving the alginate was noted with the highest frequency (100%), the genes that encoded for pilin structural subunits were noted with the lowest frequency (4%) for pil B gene, and pil A gene showed (92%). The results of the PCR detection revealed the high spread of the alg D gene, all resistant isolates contained this gene at the same time these isolates were 100% producing biofilm, alginate production is a significant factor of biofilm-associated virulence. chi-square showed a significant association between the biofilm intensity and carbapenem resistance (X² = 14.62, P < 0.023).

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“…The situation differed for the qacE gene, which was reported differently from prior Iranian studies (1.1% to 26.3%) (Table 3). The documented rate of the qacE∆1 gene was lower in Germany (10%) (22) when its reported rate was higher in other countries (Table 3) (39,(44)(45)(46)(47)(48)(49). The qacE gene frequency was lower among P. aeruginosa isolates from Germany (2.7%) (22), but several other studies reported a higher rate of qacE genes (33% -100%) (39,44,46,48,49) (Table 3).…”

Section: Discussionmentioning

confidence: 91%

High Prevalence of Antiseptic Resistance Encoding Genes and Reduced Phenotypic Antiseptic Susceptibility Among Antibiotic-Resistant Pseudomonas aeruginosa Isolates

Radmehr

Mirab-balou

Ghasemzadeh‐Moghaddam

et al. 2023

Jundishapur J Microbiol

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Background: Multi-drug resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa isolates are of clinical concern. Objectives: To determine the distribution of antiseptic resistance genes and the associated level of phenotypic antiseptic resistance against quaternary ammonium compounds and biguanide compounds, we studied MDR and XDR P. aeruginosa isolates collected from different infections among patients from a single hospital. Methods: Pseudomonas aeruginosa isolates were investigated in 2020 for in vitro susceptibility to benzethonium chloride (BTC), benzalkonium chloride (BKC), and chlorhexidine digluconate (CHG). The minimum inhibitory concentrations (MICs) against these antiseptic agents were determined using broth microdilution. Also, polymerase chain reaction (PCR)-mediated detection of qacE, qacEΔ1, and blaOXA-23 genes was used. Results: Isolates were largely non-clonal according to their phenotypical and genotypical non-similarity (35 overall data-combination types detected). Most P. aeruginosa infections occurred in intensive care unit (ICU) patients (n = 43, 61.4%). Extensively drug-resistant and MDR phenotypes were detected in 20% and 12.6%, respectively. Among the 70 isolates retained, 53 (75.7%) harbored at least one resistance gene, comprising 11 (20.7%) isolates with solely the qacEΔ1 gene; seven (13.2%) isolates harbored the qacE gene. Both the qacE and qacEΔ1 genes were detected simultaneously in 35 (66%) isolates. The mean MICs for BTC (24.0 versus 10.56 µg/mL), BKC (46.1 versus 17.22 µg/mL), and CHG (107.7 versus 29.4 µg/mL) were statistically significantly higher among antiseptic resistance gene harboring isolates than in other isolates without such genes. Conclusions: The significantly increased MICs against antiseptic agents among antibiotic-resistant P. aeruginosa isolates highlight the importance of monitoring such increases and implementing effective infection control.

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DNA Sequences of qacE∆1 Gene in Pseudomonas Aeruginosa Isolated from Wounds and Burns Infections.

Cited by 5 publications

References 10 publications

Molecular Identification of lasB gene in Pseudomonas Aeruginosa Isolated From Patients With Urinary Tract Infection

Molecular Identification of lasB gene in Pseudomonas Aeruginosa Isolated From Patients With Urinary Tract Infection

Carbapenem Resistance Related with Biofilm Formation and Pilin Genes in Clinical Pseudomonas aeruginosa Isolates

High Prevalence of Antiseptic Resistance Encoding Genes and Reduced Phenotypic Antiseptic Susceptibility Among Antibiotic-Resistant Pseudomonas aeruginosa Isolates

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