Among prevalent infectious diseases, the most frequently occurring infections are the Urinary Tract Infections (UTIs) which predominantly occur in the community as well as in the hospital settings and are one of the main cause of morbidity and mortality worldwide. Objectives: To determine the frequency of MBL E. coli species in urine samples, antibiotic susceptibility pattern and the prevalence of MDR for E. coli. Methods: There were 200 urine samples obtained from Anwar clinical laboratory Saidu Sharif, District Swat, Pakistan. Samples of urine were obtained and then cultivated in selective media i.e. Cysteine Lactose Electrolyte Deficient Agar (CLED) and MacConkey Agar Plates. In traditional morphological and biochemical studies, isolates were identified. Results: Total 58 (29.6%) positive isolates were recovered from male while 116 (70.3%) urine specimens were positive from female patients, A total isolated bacteria were MBL positive including 36 (31.3%) isolated E. coli, Furthermore, in the total isolated species were identified as MDR positive in which 80 (69.5%) were E. coli, The most potent antibiotics found against bacteria were the highest for Meropenum (78.2%), Imepenum (73.9%), and Amikacin (26.0%) Cefuroxime (21.7%), respectively and Cefaclor (19.1%) were most sensitive while antibiotic mostly resistant showed. Conclusion: This study concludes that, the most prominent bacterial isolate in the urine samples was E. coli 115 (69.6%), Carbapenem resistance is frequently observed isolates of E. coli, which indicate that MBL phenotype should be regularly determined in clinical settings to prevent emerging Carbapenem resistance.
The majority of cases with tuberculous pleuritis have negative acid-fast bacilli (AFB) on smear microscopy, making the diagnosis difficult. This case report is based on the successful diagnosis and management of an extra-pulmonary (EP) multidrug-resistant tuberculosis (MDR-TB) patient with a history of lymphoma. Initial tests revealed a right-sided pleural effusion and thickening of the pleura. The closed pleural biopsy, pleural fluid histopathology, culture, and drug sensitivity testing (DST) report revealed Mycobacterium tuberculosis with isoniazid and rifampicin resistance. Based on the DST report, the patient was labeled as a case of MDR-TB and successfully managed with an individualized drug-resistant TB (DR-TB) regimen. With initial negative microscopy and GeneXpert MTB/RIF (Sunnyvale, CA: Cepheid Inc.) reports, this case demonstrated that DR-TB could exist even in the absence of risk factors. Furthermore, it also unveils the importance of line probe assays (LPAs) and culture in identifying MDR-TB. Lymphocytic/exudative pleural effusions and pleural biopsy specimens should be subjected early on to investigations like Xpert/MTB RIF, cultures, and genotypic DST to timely diagnose and treat DR-TB.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.