Objectives:The main propose of this investigation is to evaluate the value of ultrasound irradiation technique versus boiling water bath in the reconstitution cold MIBI (Sestamibi) kits by technetium 99m. Material and Methods: Twenty Sestamibi cold kits from various batches have been chosen. The vials were randomly divided into two equal groups. The first group was reconstituted by boiling water bath as standard method and the other group reconstituted by new developed technique. The stability of radio-complex samples were examined by thin layer chromatography (ITLC) and Radio high pressure liquid chromatography (Radio-HPLC) up to 24 hours post reconstitution. The partition coefficient and protein bonding of radiotracer samples were analyzed. Results: The significant differences have not been observed in the yields and stability of radiotracer samples which were prepared by either two methods in ITLC and Radio-HPLC investigations. The partition coefficient and protein bonding assays demonstrated that radiotracer samples which were prepared by boiling water bath were a little more lipophilic than the radio-complex samples which were reconstituted by new developed technique. Conclusion: Green chemistry is convenient and efficient modality to reconstitute the cold Sestamibi kit by technetium 99m. It can be established as an alternative method to prepared 99m Tc-Sestamibi in the clinical practice.
The ultrasound irradiation technique is convenient and sufficient method to prepare (99m)Tc-sestamibi. It can be recommended as an alternative method to reconstitute sestamibi kits particularly in emergency situations to reduce potentially medical risk by avoiding any delay in acute therapy for myocardial infarction.
Background: Advanced glycation end-products (AGEs) cause proinflammatory responses and macromolecular damages. Advanced oxidation protein products (AOPPs) are protein biomarkers for oxidative stress. Levels of AGEs and AOPPs increase with the progression of chronic renal dysfunction. Objectives: In this study, we aimed to measure these species in patients with renal transplantation and to analyze their correlation with the measured glomerular filtration rate (GFR) and renal function parameters. Patients and Methods: Eighty renal transplant patients and normal subjects were recruited. GFR was measured by the two-sample plasma method with technetium-99m-labeled diethylenetriaminepentaacetic acid (TC99m-DTPA) clearance. Biochemical measurements included creatinine, cystatin C, urea, total protein, and pentosidine. Serum AGEs were determined using a fluorometric assay and AOPPs were estimated spectrophotometrically. Results: The measured GFR found to be significantly decreased in renal transplant patients compared to the control subjects (P< 0.001). Levels of AGEs, AOPPs, serum creatinine, and cystatin C were increased in renal transplant patients with lower values of measured GFR (mGFR). A significant association between the levels of AGEs species (serum fluorescence and pentosidine) and mGFR when adjusted for creatinine and other risk factors in multiple linear regression model analysis was found (P=0.05 and P=0.001, respectively). Conclusions: This study demonstrated increased levels of pentosidine and AGEs in transplant recipients were associated with decreased mGFR. Their accumulation can be predictive for the progression of chronic allograft loss of function.
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