The stability of 99mTc-MIBI (Sestamibi) complex samples which prepared under ultrasound irradiation technique versus boiling water bath method

Abstract

Objectives:The main propose of this investigation is to evaluate the value of ultrasound irradiation technique versus boiling water bath in the reconstitution cold MIBI (Sestamibi) kits by technetium 99m. Material and Methods: Twenty Sestamibi cold kits from various batches have been chosen. The vials were randomly divided into two equal groups. The first group was reconstituted by boiling water bath as standard method and the other group reconstituted by new developed technique. The stability of radio-complex samples were examined by thin layer chromatography (ITLC) and Radio high pressure liquid chromatography (Radio-HPLC) up to 24 hours post reconstitution. The partition coefficient and protein bonding of radiotracer samples were analyzed. Results: The significant differences have not been observed in the yields and stability of radiotracer samples which were prepared by either two methods in ITLC and Radio-HPLC investigations. The partition coefficient and protein bonding assays demonstrated that radiotracer samples which were prepared by boiling water bath were a little more lipophilic than the radio-complex samples which were reconstituted by new developed technique. Conclusion: Green chemistry is convenient and efficient modality to reconstitute the cold Sestamibi kit by technetium 99m. It can be established as an alternative method to prepared 99m Tc-Sestamibi in the clinical practice.

Synthesis and Evaluation of 99mTc-Tricabonyl Labeled Isonitrile Conjugates for Prostate-Specific Membrane Antigen (PSMA) Image

“…99m Tc-labeled hexakis-methoxyisobutylisonitrile ( 99m Tc-MIBI) is a lipophilic complex with a positive charge and is a well-known myocardial perfusion SPECT agent in the clinical field [40,41]. In the 99m Tc-MIBI complex, 99m Tc(I) is a soft metal and is complexed by six soft isonitrile ligands in a hexavalent fashion to form a stable octahedral complex [42]. The metal center of [ 99m Tc][Tc(CO) 3 (H 2 O) 3 ] + is likewise soft and the isonitrile ligands can strongly coordinate with it in a 3:1 ratio, as reported previously [43][44][45].…”

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“…Ultrasound waves have chemical effects on the reaction system due to the creation of cavitation or generation of free radicals which augment the rate of reaction. Green chemistry has been recommended for the preparation of technetium-99m methoxy isobutyl isocyanate ( 99m Tc-MIBI) radiotracer samples in nuclear medicine [17][18][19] . The principal aim of this investigation was to evaluate the efficacy of ultrasound irradiation technique to reconstitute the freeze-dried Octreotidekits with 99m Tc radionuclide in comparison to the boiling water bath standard method.…”

The Preparation of 99mTc-MAG3 radiotracer by Ultrasound

“…In order to carry out the radiolabeling reaction of the ligand with 99m Tc radioisotope, the ligand structure must contain functional groups that contain non-bonded electrons such as oxygen, nitrogen, sulfur, phosphorus etc, to react with empty orbitals of reduced Tc is readily performed at room temperature for radiolabeling reaction 22 . However, in some ligands, the radiolabeling reaction is facilitated by heating due to the fact the functional groups in these ligand structure do not share non-bonding electrons readily with the reduced 99m Tc [19][20][21]23,24 . Boiling water bath device is routinely used for heating source in nuclear medicine centers.…”

“…The reactions under ultrasound waves can be leaded to better yields, faster rates and milder temperatures [15][16][17][18] . The new developed technique was examined to prepare the 99m TcSestamibi radiocomplex as an alternative and reliable method in clinical practice [19][20][21] . This approach was conducted to evaluate the application of ultrasound irradiation for the reconstitution MAG 3 freeze-dried kits by 99m Tc radioisotope in comparison to the boiling water bath as a standard method.…”