Children have lower hospitalisation and mortality rates for coronavirus disease-2019 (COVID-19) than adults; however, younger children (<4 years of age)1 may develop more severe disease than older children. To date, the immune correlates of severe COVID-19 in young children have been poorly characterized. We report the kinetics of immune responses in relation to clinical and virological features in an infant with acute severe COVID-19. Systemic cellular and cytokine profiling showed initial increase in neutrophils and monocytes with depletion of lymphoid cell populations (particularly CD8+ T and NK cells) and elevated inflammatory cytokines. Expansion of memory CD4+T (but not CD8+T) cells occurred over time, with predominant Th2 bias. Marked activation of T cell populations observed during the acute infection gradually resolved as the child recovered. Significant in vitro activation of T-cell populations and robust cytokine production, in response to inactivated SARS-CoV-2 stimulation, was observed 3 months after infection indicating durable, long-lived cellular immune memory.
Background: CCR5 is identified as one of the most important chemokine receptors with a major role in the creation of chemotaxis and mobilization of immunocompetent cells and moving them toward the liver for thorough cleaning of the virus. CCR5-59353 (C/T) is an important promoter polymorphism of chemokine receptor 5. Some studies showed a relationship between CCR5-59353 (C/T) polymorphism and clearance or persistence of hepatitis B virus (HBV) infection.
Relationship between blood types and incidence and fatality of coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has not been consistent in the studies performed around the world [1-5]. Here, we investigate the association between ABO and RhD blood types with incidence and fatality of COVID-19 in the ethnically homogenous population of Babol, north of Iran. The ABO and RhD distributions of 955 RT-PCR-confirmed COVID-19 patients, hospitalized between 20 March and 21 September of the year 2020 at the affiliated hospitals of Babol university of medical sci-
Objectives
Serological methods may not be reliable for RBC antigen typing, especially in multi‐transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping.
Methods
Two hundred patients participated in this study. Blood group phenotype and genotype were determined using the serological method and PCR‐SSP, respectively. The genotypes of patients with incompatibility between phenotype and genotype were re‐evaluated by RFLP‐PCR and confirmed by DNA sequencing.
Results
Discrepancies between phenotype and genotype results were found in 132 alleles and 83 (41.5%) patients; however, there was complete accordance between the three genotyping methods. Most discrepancies were detected in Rh and Duffy systems with 47 and 45 cases, respectively, and the main discrepancy was in the FY*B/FY*B allele when serologically showed Fy(a+b+). All 39 undetermined phenotypes, due to mixed‐field reactions, were resolved by molecular genotyping.
Conclusion
Molecular genotyping is more reliable compared with the serological method, especially in multi‐transfused patients. Therefore, the addition of blood group genotyping to serological assays can lead to an antigen‐matched transfusion in these patients.
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