Nanoparticles have been applied to medicine, hygiene, pharmacy and dentistry, and will bring significant advances in the prevention, diagnosis, drug delivery and treatment of disease. Green synthesis of metal nanoparticles has a very important role in nanobiotechnology, allowing production of non-toxic and eco-friendly particles. Green synthesis of silver nanoparticles (AgNPs) was studied using pine pollen as a novel, cost-effective, simple and non-hazardous bioresource. The antifungal activity of the synthesized AgNPs was investigated. Biosynthesis of AgNPs was conducted using pollen of pine (as a novel bioresource) acting as both reducing and capping agents. AgNPs were characterized using UV-visible spectroscopy, X-ray diffraction and transmission electron microscopy. In evaluation for antifungal properties, the synthesized AgNPs represented significant in vitro inhibitory effects on cultures. Pine pollen can mediate biosynthesis of colloidal AgNPs with an average size of 12 nm. AgNPs were formed at 22 °C and observed to be highly stable up to three months without precipitation or decreased antifungal property. AgNPs showed significant inhibitory effects against. The first report for a low-cost, simple, well feasible and eco-friendly procedure for biosynthesis of AgNPs was presented. The synthesized AgNPs by pine pollen were nontoxic and eco-friendly, and can be employed for large-scale production. The nanoparticles showed strong effect on quantitative inhibition and disruption of antifungal growth.

The increasing resistance to macrolide, lincosamide, and streptogramin B agents among methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem for the health community. This study aimed to investigate the prevalence of ermA, ermB, ermC, and msrA in MRSA strains isolated from burn patients in Ahvaz, southwest of Iran. A total of 76 isolates of S. aureus were collected from January to May 2017 from Taleghani Burn Hospital in Ahvaz. Among 76 S. aureus strains collected, 60 (78.9%) isolates were MRSA. The antimicrobial susceptibility testing for MRSA showed extreme high resistance rate to clarithromycin (100%) and azithromycin (100%), followed by erythromycin (98.3%). The PCR assay revealed that the frequency rates of msrA, ermA, and ermC genes were 23 (38.3%), 28 (46.7%), and 22 (36.7%), respectively. In addition, none of the MRSA isolates had the ermB gene. Because of the high prevalence of macrolide and lincosamide resistance found in MRSA isolates from infections of burn patients in Ahvaz, southwest of Iran, it is recommended that local periodic survey be performed for controlling the dissemination of antimicrobial resistance.

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Background:According to the studies, many pathogens function as cofactors interacting with Human papillomavirus in the development of pre-cancer or cancer of the cervix. The aim of this study was to investigate the prevalence rate of Sexually Transmitted Infections (STIs) pathogens including Mycoplasma hominis, Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae, Gardnerella vaginalis, and Streptococcus agalactiae in people with HPV and without HPV infection, and frequency rate of these pathogens in high and low risk of HPV. Methods: Cervical samples of 280 women who referred to Tehran west hospitals in Iran, between 2019 and 2020, were collected. After DNA extraction of samples, identification of HPV and genotyping was performed, and then, to detect each microorganism, the PCR was carried out with specific primers. Finally, the results were analyzed using descriptive statistics tests. Results: The mean age of patients was 37 years. Two groups of patients were identified based on positivity or negativity of HPV. In HPV-positive group (118 cases), the prevalence of U.

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Background and Objectives: The pentose phosphate pathway is of particular importance for energy supply in red blood cells. Glucose 6-phosphate dehydrogenase is the key enzyme involved in this pathway. The deficiency of this enzyme due to lack of nicotinamide adenine dinucleotide phosphate (NADPH) production in red blood cells leads to oxidation, hemoglobin deposition, red blood cell membrane changes and favism, which causes jaundice and hemolytic anemia in newborns. In this study, we evaluate the frequency of G6PD deficiency in newborns in a hospital in Bam, Iran. Methods: In this descriptive-analytical cross-sectional study, blood samples were taken from 572 newborns hospitalized at Pasteur hospital of Bam (Iran) in the first half of 2018. Demographic data including gender and blood type were collected. The G6PD enzyme activity was evaluated using qualitative G6PD assay kit (Saba Teb, Iran). Data were analyzed using SPSS software. Results: Of the 572 cases examined, 52 (9.09%) had G6PD deficiency. Of the affected patients, 34 (65.4%) were male and 18 (34.6%) were female. In addition, the majority (47.7%) of affected patients were with O + blood group. Conclusion: A relatively high frequency of G6PD deficient infants was reported in Bam. As expected, the prevalence of this disorder was higher in males than in females. Given the lost cost of screening, we suggest screening for G6PD enzyme activity in all newborns.