Bone graft material should possess sufficient porosity and permeability to allow integration with native tissue and vascular invasion, and must satisfy oxygen and nutrient transport demands. In this study, we have examined the use of three-dimensional (3D)-printed polycaprolactone/tricalcium phosphate (PCL/TCP) composite material in bone grafting, to estimate the scope of its potential application in bone surgery. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) are known to enhance osteointegration. We hypothesized that a patient-specific 3D-printed solid scaffold could help preserve seeded ADSCs and BMSCs and enhance osteointegration. Diffuse osteogenic tissue formation was observed by micro-computed tomography with both stem cell types, and the ADSC group displayed similar osteogenesis compared to the BMSC group. In histological assessment, the scaffold pores showed abundant ossification in both groups. Reverse transcription polymerase chain reaction (RT-PCR) showed that the BMSC group had higher expression of genes associated with ossification, and this was confirmed by Western blot analysis. The ADSC- and BMSC-seeded 3D-printed PCL/TCP scaffolds displayed promising enhancement of osteogenesis in a dog model of maxillary bone defects.
Background and Objectives Facial rejuvenation via mesotherapy using a new polycomponent formulation was employed to improve skin texture. In this study, we evaluated the rejuvenating effects of a new polycomponent formulation through mesotherapy using a microneedle therapy system (MTS). Materials and Methods Twenty patients underwent a series of facial procedures, including three sessions performed at 2-week intervals and two sessions at 4-week intervals. Subsequently, 3 mL of new polycomponent formulation was applied to the entire face and MTS was used. Patient satisfaction scoring was conducted by three plastic surgeons who evaluated the improvement of fine wrinkles, skin tightness, hydration, and sweat pore size. In addition, photographs of the patients were taken at each visit and 2 months after the last treatment session. Results The patients were satisfied with the therapeutic outcomes. The scores of the surgeons for the improvement of fine wrinkles, skin tightness, hydration, and sweat pore size were high. Conclusion Minimally invasive mesotherapy using a new polycomponent formulation can improve the clinical appearance of facial skin. However, a largerscale and well-controlled investigation of the clinical efficacy and safety of this technique is needed to confirm these findings.
Background/Aim: The skin protects the body from ultraviolet rays and other external factors. Various studies have been conducted to identify methods to prevent skin aging and damage. To investigate the protective effects of methylsulfonylmethane (MSM), in this study, a hairless mouse model was used. Patients and Methods: Mice divided into Groups B, C, and D were subjected to UVB irradiation for six weeks, and Group A was considered the control. Retinoic acid is a substance that has been proven to have anti-aging properties. Group C was injected with MSM, group D was injected with retinoic acid, and groups A and B were injected with saline. At the end of the experiment, the degree of senescence was confirmed through visual evaluation, histopathological analysis, immunohistochemistry, and elasticity measurement using SEM. Results: After the end of the experiment, the wrinkle score was 0.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.