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Background In recent years, targeted cancer treatment methods at various molecular levels have been developed for non-small cell lung cancer (NSCLC), one of two major subtypes of lung cancer. miRNA-based clinical trials are currently the preferred targeted therapeutic strategy. Also, ceRNAs (competing endogenous RNA) would be the newest and the most effective approach to uncover novel interactions between mRNAs and miRNAs in NSCLC carcinogenesis. There are many factors influencing on efficiency of a miRNA to suppress or silence translation of the target mRNA. The most effective event is the presence of other RNAs showing ceRNA activity. These RNAs contain binding sites for specific miRNAs and enable miRNAs to bind these pseudo targets, instead of the original binding sites on the target mRNA. Therefore, the mRNA of the target gene is less affected by this miRNA, while the amount of miRNA remains the same in the media. Method For this project, we determined that five clinically important different oncogenes (PDL1, FGFR1, DDX3X, SLC1A5, FXR1) are involved in the pathogenesis of NSCLC. For this purpose, we transfected model NSCLC cell line, A549, with miRNAs (miR-150-5p, miR-15a-5p, miR-503-5p) targeting these oncogenes to investigate whether these oncogenes will be suppressed at the mRNA level and also how the suppression efficiency of these miRNA on the oncogenes will be affected by possible ceRNA (CNKSR3, POU2F1, HIPK2) activities. Results miR-15a-5p was determined to have the most suppressive effect on the five genes and three potential ceRNAs (p<0.05). Furthermore, CNKSR3 was the ceRNA most affected by all three miRNAs (p<0.05). Conclusion CNKSR3 was affected more than the oncogenes known to act on NSCLC and this might make it a stronger and novel marker for use in possible treatment regimens designed using miR-15a-5p silencing effect on oncogenes in NSCLC pathogenesis. According to literature, this is the first study associating NSCLC with miR-15a-5p and CNKSR3.

The title complexes [M(sac)(2)(mpy)(2)] [sac is saccharinate (C(7)H(4)NO(3)S) and mpy is 2-pyridylmethanol (C(6)H(7)NO)], with M = Zn(II) and Cd(II), are isostructural and consist of neutral molecules. The Zn(II) or Cd(II) cations are octahedrally coordinated by the two neutral mpy and two anionic sac ligands. The mpy ligand acts as a bidentate donor through the amine N and hydroxyl O atoms. The sac ligands exhibit an ambidentate coordination behaviour; one is N-coordinated and the other is O-coordinated within the same coordination octahedron. The crystal packing is determined by C-H...O-type hydrogen bonding, as well as by weak py-py and sac-sac aromatic pi-pi-stacking interactions.

New lead(II)-saccharin complexes, [Pb(sac) 2 (pym)] (1) and [Pb(sac) 2 (pydm)] (2) (sac ϭ saccharinate anion; pym ϭ 2-pyridylmethanol; pydm ϭ pyridine-2,6-dimethanol) were synthesized and characterized by IR spectroscopy and single crystal X-ray diffractometry. Complex 1 crystallizes in the monoclinic P2 1 /c space group with Z ϭ 4, while the crystals of complex 2 are extremely X-ray sensitive and decompose by the X-ray beam within one day. Pym and pydm act as bi-and tridentate ligands, respectively. Most important feature of the complexes is non-equivalent coordination of the sac ligands to the lead(II) atom. In the complex 1, the sac ligands coordinate to the lead(II) ion in two distinct manners. One

Bis(pyridine-2,6-dimethanol-N,O,O H)cobalt(II) disaccharinate dihydrate, [Co(C 7 H 9 NO 2) 2 ](C 7 H 4 NO 3 S) 2 Á2H 2 O, (I), and bis(pyridine-2,6-dimethanol-N,O,O H)copper(II) disaccharinate dihydrate, [Cu(C 7 H 9 NO 2) 2 ](C 7 H 4 NO 3 S) 2 Á2H 2 O, (II), collectively [M(dmpy) 2 ](sac) 2 Á2H 2 O (where M is Co II or Cu II , sac is the saccharinate anion and dmpy is pyridine-2,6-dimethanol), are isostructural. The [M(dmpy) 2 ] 2+ cations exhibit distorted octahedral geometry in which the two neutral dmpy species act as tripodal N,O,O H-tridentate ligands. The crystal packing is determined by hydrogen bonding, as well as by weak pyridine±saccharinate %±%-stacking interactions. Comment The arti®cial sweetener saccharin (also known as 1,2benzisothiazol-3(2H)-one 1,1-dioxide or o-benzosulfimide) is readily deprotonated to form the corresponding saccharinate anion (sac). The coordination behaviour of sac to metal ions depends on the presence of other co-ligands. Bulky ligands,

Synthesis, crystal structure, spectroscopic and thermal properties of trans-[Ni(sac) 2 (aeppz) 2 ] [sac = saccharinate and aeppz = N -(2-aminoethyl)piperazine], A nickel(II) complex of saccharinate (sac) with N-(2-aminoethyl)piperazine (aeppz), trans-[Ni(sac) 2 (aeppz) 2 ], has been prepared and characterized by elemental analyses, IR, UV-vis, magnetic measurements and single crystal X-ray diffraction. The compound crystallizes in space group P 1 1 (No. 2). The nickel(II) ion sits on an inversion centre and is octahedrally coordinated by two neutral aeppz and two anionic sac ligands. The aeppz ligand is bidentate (N,N 0 ) chelating, while sac is O-coordinated through the carbonyl O atom. The IR spectrum of [Ni(sac) 2 (aeppz) 2 ] displays typical absorption bands of bidentate aeppz and O-bonded sac ligands. Under atmospheric conditions, [Ni(sac) 2 (aeppz) 2 ] is thermally stable up to 2208C, but at higher temperatures, loses two aeppz ligands between 220 and 3178C and two sac ligands in the temperature range 320-5288C, giving NiO as end product.

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