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An electrochemical sensing platform based on composite material, consisting of molecularly imprinted polymer coated on graphene oxide (MIP‐GO), was developed for selective and sensitive analysis of amoxicillin (AMOX). The MIP‐GO composite, which was fabricated by sol‐gel polymerization after removal of template molecule, was deposited as a thin film on glassy carbon electrode, and then was electrochemically characterized by cyclic voltammetry and differential pulse voltammetry. The linear response for the determination of AMOX was obtained in the concentration range from 5.0×10−10 to 9.1×10−7 M under the most proper conditions and the detection limit was found to be 2.94×10−10 M.