Recently resveratrol, a compound naturally occurring in various plants, has been proposed as a potential anti-obesity compound. The aim of the present work was to analyse the effects of different doses of resveratrol on body fat and serum parameters in rats. Thirty-two male Sprague-Dawley rats were randomly divided into four groups and fed on a hypercaloric diet for 6 weeks. The doses oftrans-resveratrol used were 6, 30 and 60 mg/kg body weight/d in RSV1, RSV2 and RSV3 groups respectively. The stability of resveratrol when added to the diet was evaluated. Blood samples were collected, and white adipose tissue from different anatomical locations, interscapular brown adipose tissue, gastrocnemious muscles and liver were weighed. Commercial kits were used to measure serum cholesterol, glucose, triacylglycerols and non-esterified fatty acids. While the lowest dose did not have a body fat reducing effect, the intermediate dose reduced all the white adipose depots. The highest dose significantly reduced mesenteric and subcutaneous depots but not epididymal and perirenal tissues. Although the reduction in all the anatomical locations analysed was 19% in the RSV3 group, in the RSV2 group it was 24%. No significant differences among the experimental groups were found in brown adipose tissue, gastrocnemious muscle or liver weights. Serum parameters were not affected by resveratrol intake because no differences among the experimental groups were observed. These results suggest that resveratrol is a molecule with potential anti-obesity effect. The most effective of the three experimental doses was 30 mg/kg body weight/d.

RESUMOHá alguns relatos na literatura sugerindo associação entre polimorfismos do receptor b 2 -adrenérgico com obesidade e outros com hipertensão arterial. O objetivo do nosso estudo foi estudar a freqüência de um polimorfismo do receptor b 2 adrenérgico (Gln27Glu) em pacientes obesos (BMI 48 ± 8,2kg/m 2 ) e relacioná-lo com hipertensão arterial, e níveis de triglicérides, colesterol, insulina e glicose no sangue. Encontramos associação deste polimorfismo em obesos com hipertensão arterial. The sympathetic nervous system activation stimulates energy mobilization and utilization in the adipose tissue that is a favored target for high-energy substrate storage, mobilization and utilization. Adrenergic responsiveness may be altered in obesity and could be an important factor in the pathogenesis and maintenance of obesity state. In the hypertensive state there is physiological and biochemical evidence that b-adrenergic responsiveness is diminished in the face of increased sympathetic tone. Recently, several different polymorphic forms of the human b 2 AR have been identified in general population, including N-terminal substitutions of glutamine (Gln) for glutamic acid (Glu) at position 27. The aim of this study was to investigate the potential interaction between the b 2 AR (Gln27Glu) polymorphism and obesity accumulation and hypertension in morbidly obese subjects. The Ita I genotypes of b 2 AR were established using RFLP methods in 135 individuals with BMI 48 ± 8.02kg/m 2 . The frequency of Gln/Glu was 31.9% and in the homozygous Glu/Glu was 12.6%. No association was found between BMI, weight gain during the past years and the Ita I genotypes and neither was associated with levels of triglycerides, cholesterol, insulin and glucose. Positive association was found between blood pressure (systolic and diastolic) and presence of polymorphism. The results indicate at the first time that presence of polymorphism 27Glu may provide a mechanism for enhanced vascular reactivity and identify a candidate gene for hypertension in this obesity group. Na última década, a comunidade científica tem procurado estabelecer o papel da genética na etiologia da obesidade humana e de outras doenças complexas que têm componentes ambientais, comportamentais e genéticos. Estuda-se em que extensão os genes herdados têm influência no

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