Objectives To determine the effects of long-term cigarette were no significant changes in testosterone in the control rats. The mean plasma LH and FSH levels of smoking on the levels of plasma testosterone, luteinizing hormone (LH) and follicle-stimulating hormone the two groups did not change significantly after exposure. In rats exposed to smoke, histological exam-(FSH) in male adult rats and to examine morphological and histological changes in the testes.ination of the testes showed fewer Leydig cells and degeneration of the remaining cells. Materials and methods Cigarette smoke was generated by a smoking-machine and 12 rats were exposed to Conclusion These results indicate that the decrease in plasma testosterone levels induced by exposure to cigarette smoke diluted with 90% air for 60 days (2 h/day). Twelve rats were exposed to room air only smoke was not associated with changes in plasma gonadotrophin levels. The decrease in testosterone under similar conditions as controls. The concentrations of plasma testosterone, LH and FSH were levels may be related to the toxic effects of smoke on Leydig cells. measured before and after exposure using a radioimmunoassay and the testes were examined
Exposure to tobacco smoke can cause irritation of the conjunctiva. We conducted this study to identify the effect of tobacco on rat conjunctiva. Animals were divided into experimental and control groups and we exposed the experimental group to tobacco smoke. Control group rats inhaled only room air. Spectrophotometric analysis of the smoke-air mixture revealed that many toxic substances were present in this compound. We found very high levels of plasma thiocyanate after exposure to smoke in experimental group animals but no increase in the control group. So, this data indicates that these animals inhaled smoke effectively in our method. After 3 months conjunctivas were examined by light and electron microscopy. In the experimental group, conjunctivas were thinned, atrophied and microvillous projections and desmosomal connections were absent in comparison with the control conjunctivas. This pathologic change is very similar to conjunctival response to chronic irritants of any type.
Background: It is a well-established fact that cigarette smoking causes degenerative, inflammatory, and respiratory diseases in humans.Because many factors such as air pollution and harsh working conditions can easily be eliminated in animal studies, we conducted this study to identify the effect of tobacco on rat trachea. Methods: 24 male Wistar rats were divided randomly into an experimental and a control group. The experimental group of rats was exposed to cigarette smoke for 2 h each day over a duration of 60 consecutive days and the control group was treated in an identical fashion yet exposed only to room air. A morphometric study was performed on tracheal specimens taken from 22 rats (10 smoke-exposed rats and 12 control rats). Results: Our results show that many of the morphological changes of the tracheal epithelium were found in the experimental group and significant quantitative differences were observed between the two groups. Loss of cilia, basal cell hyperplasia, goblet cell hyperplasia and an increased number of subepithelial inflammatory cells were observed by light microscopic examination of the trachea of experimental rats. We found very high levels of plasma thiocyanate after exposure to smoke in the experimental group, but no increase in the control group. Conclusions: The oxidants contained tobacco which could play an important role in the development of these structural and functional abnormalities in the trachea after smoke exposure. In addition, smoking can recruit inflammatory cells to the trachea.
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