Tooth autotransplantation refers to tooth transplantation from the donor to the recipient site of the same individual. Occasionally, there are situations where autotransplantation cannot be carried out immediately at clinic. In such cases, donor teeth cryopreservation is necessary to overcome limited indications. Cryopreservation media and nutrients play essential roles in preserving the periodontal ligament cells' activity. Several studies have focused on cryopreservation media and nutrients. Platelet-rich plasma (PRP) contains lots of growth factors without inducing immunologic responses or pathologic complications. Therefore, self serum and PRP might be positive factors in the cryopreservation process for preserving the periodontal ligaments (PDLs) viability. In this study, beagle dog's incisors were cryopreserved for three months in different cryopreservation media, including Dulbecco's modified eagle medium (DMEM, containing DMSO), DMEM+20% self serum, DMEM+20% self serum+PRP, and DMEM+20% fetal bovine serum (FBS). Histological results revealed that DMEM+20% self serum+PRP and DMEM+20% FBS groups showed significantly less root resorption and higher regeneration of periodontal ligament cells and that PRP is a positive factor in the cryopreservation process to preserve PDLs viability. Therefore, cryopreservation media with PRP will be a good periodontal tissue regeneration option after long-term cryopreservation in a tooth autotransplantation procedure.
The current study introduces photocurable macromolecular resin (KIS resin) and aims to assess its use as a basic material for implant surgical stent fabrication using 3D printing and the digital light processing (DLP) technique. The biological and physical properties of KIS resin were evaluated, and radiological analysis was performed to assess the clinical accuracy of a 3D-printed surgical stent. The physical characteristics were analyzed in accordance with the guidelines of certified standards, and cell adhesion analysis was performed to evaluate the viability of human gingival fibroblast (hGF) cells exposed to KIS resin and conventional orthodontic acrylic resin. Radiological analysis of animals was carried out by pairwise overlap of pre-and postoperative images. The physical characteristics of KIS resin met the ISO 20795-1, ASTM D695, and ASTM D638 standards. The hGF cells on KIS resin samples exhibited better cell attachment and connection compared to those exposed to conventional resins. The findings of the radiological analyses showed statistically significant differences in the deviations between DLP-printed surgical stents and conventional stents, with the horizontal linear deviations being smaller in the coronal and middle levels of the former. Moreover, the angular deviation was also smaller in the DLP stent group. Therefore, KIS resin exhibited favorable physical properties and cell viability, highlighting its potential for use as a basic material for 3D-printed implant surgical guides. Further evaluation of the use of implant surgical stents fabricated using KIS resin and the DLP method as a proper tool for implant placement with clinical utility should be carried out.
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