Multienzymatic cascade reactions are a most important technology to succeed in industrial process development, such as synthesis of pharmaceutical, cosmetic, and nutritional compounds. Different strategies to construct multienzyme structures have been widely reported. Enzymes complexes are designed by three types of routes: (i) fusion proteins, (ii) enzyme scaffolds, or (iii) immobilization. As a result, enzyme complexes can enhance cascade enzymatic activity through substrate channeling. In particular, recent advances in materials science have led to syntheses of various materials applicable for enzyme immobilization. This review discusses different cases for assembling multienzyme complexes via random co-immobilization, compartmentalization, and positional co-immobilization. The advantages of using immobilized multienzymes include not only improved cascade enzymatic activity via substrate channeling but also enhanced enzyme stability and ease of recovery for reuse. In this review, we also consider the latest studies of different model enzyme reactions immobilized on various support materials, as multienzyme systems allow for economical product synthesis through bioprocesses.
This study details the preparation and application of supramolecular host−guest inclusion complexes entrapping biomineralized microspheres for long-term storage and their pHresponsive behavior. The microspheres were assembled using a CaCO 3 synthesis process coupled with cyclodextrin−tetrahydrocurcumin (CD−THC) inclusion complexes, forming fine-textured and mechanically stable hybrid materials. The products were successfully characterized using field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and particle size analysis (PSA). Various parameters such as the Brunauer−Emmett−Teller (BET) surface area, single point total pore volume, and pore size via adsorption/desorption analysis were also determined. The obtained THC-entrapped hybrid microspheres contained as high as 20 wt % THC loading and were very stable, preserving 90% of the initial concentration over four weeks of storage at different temperatures, largely limiting THC leaching and indicating high stability in a physiological environment. In addition, the pHresponsive release of THC from the hybrid microspheres was observed, showing potential use for application to weakly acidic skin surfaces. To our knowledge, this is the first demonstration of antiaging cosmetic formulation technology using biomineralization based on the co-synthesis of CaCO 3 and CD−THC complexes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.