Kanker kolorektal merupakan salah satu penyakit penyebab kematian tertinggi didunia yang disebabkan oleh berbagai faktor seperti mutasi genetik, epigenetik, disregulasi oleh jalur sinyal komunikasi dan mikrobiota usus. Adanya mutasi genetik dan epigenetik menyebabkan hilangnya ekspresi dan fungsi dari PTEN (Phosphatase and TENsin homolog) dimana aktivasi yang terus menerus dari kaskade pensinyalan intraseluler PI3K/Akt yang terlibat dalam karsinogenesis kolorektal. Penanganan masalah tersebut yaitu menggunakan miRNA-21 yang berperan penting dengan menekan gen PTEN penanda biologis yang berguna untuk diagnosis dan pengobatan pada kanker kolorektral. Penelitian bertujuan untuk menentukan pengikatan spesifik miRNA-21 dengan gen PTEN yang berperan dalam kanker kolorektal berdasarkan nilai ∆Ghybrid dan Logistic Probability. Sekuens lengkap gen PTEN diperoleh dari FASTA NCBI dan data CDS diperoleh dari Refseq. Prediksi pengikatan miRNA-21 matur dengan gen PTEN dilakukan menggunakan website StarMir Sfold 2.2. Hasil prediksi pengikatan miRNA-21 dengan 1 target diperoleh 296 model pengikatan. Pengikatan spesifik dilihat dari ∆Ghybrid -23,600 dan logistic probability 0,818 antara ikatan gen PTEN dengan has miR-21-5p pada CDS seedless posisi 3627-3653 dibuktikan dengan nilai ∆Ghybrid paling rendah dari semua ikatan yang terjadi.
Obesity has now become an epidemic health problem throughout the world. Its prevalence is increasing not only in Indonesia but in several other developing countries besides that, it is also increasing in several developed countries. An excessive sedentary lifestyle can cause health problems in the form of obesity. Determine the relationship between a sedentary lifestyle and the incidence of obesity in elementary school-aged children at SDN 2 Terang, Bulukumba Regency. This research is a type of analytic observational research with a cross-sectional approach, the total population is 103 students, with a total sample of 50 students consisting of class VI SD, with sampling using the probability sampling method using a sampling technique, namely random sampling. The results of this study indicate that the value of p = 0.485, so there is no significant relationship between the relationship between a sedentary lifestyle and the incidence of obesity in elementary school-age children at SDN 2 Terang-Terang, Bulukumba Regency. In this study, it can be concluded that sedentary life is high in respondents who are not obese and there is no significant relationship between sedentary lifestyle and the incidence of obesity in students of SDN 2 Terang-Terang, Bulukumba Regency, while the suggestion in this study is that elementary school-age children need awareness to better understand the things that can interfere with health problems.
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