Novel cell culture substrates for non-invasive-targeted cell recovery were developed by grafting an upper-critical-solution-temperature (UCST)-type poly(acrylamide-co-acrylonitrile) (PAA) onto a glass substrate as a cell attachment scaffold by surface-initiated reversible addition-fragmentation chaintransfer (Si-RAFT) polymerization. To optimize the UCST of PAA, the cloud point of the free polymer was first determined. Varying the chain lengths of PAA with an optimized AN-compositions of 31 mol% followed by Si-RAFT produced immobilized glass substrate (PAA-g). The PAA-g surface became hydrophilic, and the polymer brushes swelled as the temperature increased from 30 to 38 C. In addition, the viscoelasticity of PAA-g was increased by the polymer brushes swelled. Thermal detachment of PAA-g attached human immortalized mesenchymal stem cells (hiMSC) was observed by raising the temperature from 30 to 38 C. The polymer brushes length of PAA-g affects the degree of hiMSC detachment, likely due to the change in viscoelasticity with the polymer brushes length.
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