Preparation of poly(acrylamide‐co‐acrylonitrile) grafted glass and thermal stimulated detachment of surface‐attached human immortalized mesenchymal stem cells

Abstract

Novel cell culture substrates for non-invasive-targeted cell recovery were developed by grafting an upper-critical-solution-temperature (UCST)-type poly(acrylamide-co-acrylonitrile) (PAA) onto a glass substrate as a cell attachment scaffold by surface-initiated reversible addition-fragmentation chaintransfer (Si-RAFT) polymerization. To optimize the UCST of PAA, the cloud point of the free polymer was first determined. Varying the chain lengths of PAA with an optimized AN-compositions of 31 mol% followed by Si-RAFT produced immobilized glass substrate (PAA-g). The PAA-g surface became hydrophilic, and the polymer brushes swelled as the temperature increased from 30 to 38 C. In addition, the viscoelasticity of PAA-g was increased by the polymer brushes swelled. Thermal detachment of PAA-g attached human immortalized mesenchymal stem cells (hiMSC) was observed by raising the temperature from 30 to 38 C. The polymer brushes length of PAA-g affects the degree of hiMSC detachment, likely due to the change in viscoelasticity with the polymer brushes length.

Preparation of zwitterionic random and block poly(N-α-acrylamide-l-lysine-co-2-methacryloyloxyethyl phosphorylcholine) copolymers and their effect on fibrinolytic activity

“…Surface treatment of glass substrates was performed according to a previous report. 25 Scheme 3 shows the procedure for immobilization and graft polymerization of the initiator onto the glass substrate. Matsunami micro cover glass (thickness = 0.12–0.17 mm) was immersed in Piranha solution [30% H 2 O 2 : H 2 SO 4 = 1 : 3 (v/v)] for 1 h at room temperature.…”