Serum autoantibodies to thyroid peroxidase (TPO) in patients with thyroid autoimmune diseases were studied by micro-ELISA and immunoblotting. Twenty-four patients, 15 with Graves' disease and 9 with Hashimoto's thyroiditis, whose serum titers were greater than 3200 on the microsomal hemagglutination test (except for 1 patient with a titer of 800) had autoantibodies to TPO. Both immunoglobulin G and M classes of autoantibodies were detected, with the former being more prominent. When TPO and thyroid microsomes were used as a target in a competitive binding inhibition test, the results suggested that TPO was a major thyroid microsomal antigen. On the other hand, immunoblotting analysis showed 3-4 bands in the 45-60K region stained by patients' sera in addition to human TPO with mol wt of 100K and 107K; only the latter 2 bands stained with antiporcine TPO antibody. In the majority of sera, TPO bands were clearer than others, although some sera showed the clearest band with a mol wt of 55K. These results indicate that patients with autoimmune thyroid disease often have autoantibodies to TPO that can be detected by micro-ELISA and immunoblotting, and that TPO is a major component of the thyroid microsomal antigen.

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We examined the in vivo expression of IL-1b and its transcript after cerebral ischemia produced in mice cardiac arrest model. The IL-1b mRNA in the hippocampal region reached a detectable level at 1 hr after ischemia and had a peak at 3 hr after ischemia recirculation. But it was markedly decreased at 1 day and reached the control levels at 4 day after ischemia recirculation. The IL-1b-like immunoreactivity was observed at 1, 2, 4 day after ischemia recirculation and its immunoreactivity was detected at 2 day. The IL-1b-like immunoreactivity was observed in both microglia and astrocytes after bran ischemia by double immunostaining. These results provide the direct evidence for the localization and induction of IL-1b expression in vivo in mice after ischemia. It is suggested that IL-1b, produced in both astrocytes and microglia cells after ischemia, directly affect on neurons as well as glial cells to induce delayed neuronal cell death.

A "simultaneous" enzyme-linked immunoassay for the measurement of anti-thyroglobulin autoantibody in human serum was studied, in which human thyroglobulin-coated silicone rubber rod, 8-Dgalactosidase-or horseradish peroxidase-conjugated human thyroglobulin and serum sample were mixed at the same time.I n order to obtain a suitable range of the measurable antibody, an 1303