BackgroundThe full-thickness articular cartilage defects of knee have a poor healing capacity that may progress to osteoarthritis and need a knee replacement. This study determines the healing effect of bioglue in fullthickness articular cartilage defect of femoral condyle in rabbit.MethodsForty-eight male rabbits were randomly divided into four equal groups. In group A, 4 mm articular cartilage defects were created in the right and left medial femoral condyles. Then a graft from xiphoid cartilage was transferred into the defect together with a designed bioglue and the knees were closed. In group B, an articular cartilage defect was created identical to group A, but the defect size was 6 mm. In group C, 4 and 6 mm articular cartilage defects were created in the right and left medial femoral condyles respectively. The graft was transferred into the defect and the knees were stitched. In group D, articular cartilage defects were created similar to group C, just filled with bioglue and closed. The rabbits were euthanized and subgroups were defined as A1, B1, C1 and D1 after 30 days and A2, B2, C2 and D2 after 60 days. The cartilages were macroscopically and histologically investigated for any changes.ResultsMicroscopic and macroscopic investigations showed that bioglue had a significant healing effect in the femoral condyle.ConclusionAddition of bioglue can effectively promote the healing of articular cartilage defects.

Background Liver cirrhosis is a chronic disease in which normal liver tissue is replaced by fibrous tissue, leads to liver malfunction. Although transplantation is the most certain cure, stem cell therapies are shedding light on efforts to regenerate cirrhotic liver. The purpose of this study was to evaluate the regenerative potential of mesenteric fat stem cells in CCL4-induced liver cirrhosis in an animal model. Methods Thirty rats were treated with the mixture of CCL4 and olive oil intraperitoneally by a dose of 0.2 ml (0.1 ml CCL4 and 0.1 ml olive oil) every other day for 16 weeks till cirrhosis signs appeared. Fifteen rats were randomly selected as control group. Others treated by mesenteric fat derived mesenchymal stem cells transferred into the liver parenchyma. Results After 5 weeks, rats received stem cells had improved clinically by increased movements, appetite, improvement in overall behavior and decreased abdomen size. Histopathologically, liver cells showed state of regeneration and forming new colonies. Conclusion Liver cirrhosis was induced. The mesenchymal stem cells derived from mesenteric adipose tissue could improve hepatic status of the rats, as cirrhotic livers were regenerated back into normal appearing parenchyma. Rats’ clinical behavior also reached healthy status.

Findings suggest that in this study, laser treatment did not enhance callus formation nor reduce repair time of complete fracture of the radius in rabbits.

The tubular, pneumatic long bones of the bird present unique challenges in veterinary orthopedics. Various traditional and innovative forms of fracture fixation have been applied in bird orthopedic. The purpose of this study was to compare the applicability of the stainless steel intra-medullary pin with the pins made from ovine and canine long bones. Ovine and canine long bones were used to prepare pins in appropriate size and dimension. The pins were treated chemically, hydrated, dried, packaged and sterilized by ethylene oxide. Forty domestic male pigeons were divided into four equal groups. Pigeons were anesthetized by combination of xylazine and ketamine. Humeral bones were cut by a diamond disc burr. In the first group the fractured bones were not treated and was regarded as control group. In the second group stainless steel pins were inserted into the humeral medulla of pigeons; and in the third and fourth groups, prepared pins from ovine and canine long bones were inserted into the medulla, respectively. The operated wing was bandaged to the body and stabilized. Post-operative care included: clinical assessment of surgical wound, wing holding and flight assessment. The flight behavioral assessment and radiographic studies were carried out every two weeks for 32 weeks.After 32 weeks the pigeons of the first group were not able to fly, in the second group pigeons had imbalance in flight; and in the third and fourth groups the pigeons were able to fly with no problem. Radiographic study showed no significant differences between groups 2, 3 and 4 but there was a significant difference between group 1 and other three groups. None of the bone pins were rejected; all were absorbed in later stages in large quantities.The IM pins made from long bones of sheep and dog pins can be considered as an appropriate and alternative internal fixation technique, because they are very firm, strong, provide very good internal fixation for bone alignment, showing no tissue sensitivity, no rejection and therefore not necessity to be removed. While the stainless steel IM pin creates imbalance in flight and they have to be removed after bone union is completed, which needs another surgical intervention and stress.

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Background:The tendon repairs slowly. Platelets' alpha granules contain polypeptide growth factors that stimulate proliferation of normal connective tissue cells. The acute phase of inflammation is necessary for healing progress and fibroplasia. Platelet growth factors initiate and accelerate the inflammatory response by the host. Objectives: This study aimed to investigate if platelet-rich plasma (PRP) helps tendon gap healing. Methods: Aseptic surgery was performed on 24 mature guinea pigs in four groups to excise a 7-mm piece of the deep digital flexor (DDF) tendon of the right hind leg. In groups 1 and 2, the two ends of the severed tendon were sutured in the fascia conduit; group 2 received PRP injection into the conduit. In groups 3 and 4, the two ends of the severed tendon were sutured in a silicone tube conduit, and group 4 received PRP injection. After 42 days, the animals were euthanized and tendon samples were collected for further study. Results: The histopathological and stereological data showed that inflammation was far less in PRP-treated groups as they showed more fibroblastic proliferation. The synergistic effects of silicone tube and PRP injection showed the least inflammatory response. The diameter of collagen bundles and their volume were higher in the groups which received PRP in the silicone conduit. Conclusions: The present study found that PRP improved healing of tendon injury and silicone conduit served as good surgical technique with synergistic effect beside PRP.