Alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin E activity in humans. Most cultivated soybean seeds contain γ-tocopherol as the predominant form, and the ratio of α-tocopherol content to total tocopherol content (α-tocopherol ratio) is <10%. Three soybean accessions from Eastern Europe have α-tocopherol ratios of >20%. This higher content is likely due to mutations in the promoter region of the γ-tocopherol methytransferase-3 (γ-TMT3) gene. We surveyed a wild soybean germplasm collection and detected 16 accessions with stable seed α-tocopherol ratios of >20% under different growth conditions. The α-tocopherol ratios were greatly reduced when the plants were grown under cool temperatures during seed maturation, but increased to varying degrees at higher temperatures. Sequence analysis of the γ-TMT3 promoter of 11 of the accessions identified four haplotypes, one of which corresponded to that of cultivars with higher contents. These wild accessions can thus serve as novel donors for breeding cultivars with high α-tocopherol ratios and for better understanding the genetic basis of α-tocopherol synthesis in soybean.
This study was conducted to examine the effect of feeding levels of concentrate feed on growth performance, meat quality and economic evaluation of Korean black goats. The 40 male Korean native goat were divided into four treatment groups T1 (1.5%), T2 (2.0%), T3 (2.5% feeding of concentrate feed body weight per day) and T4 (ab libitum) with ab libitum rice straw. Rice straw intake decrease as against feeding levels, however average daily gains were 22, 50, 69 and 94 g/day in T1, T2, T3 and T4 groups respectively levels (p<0.05). Dressing percentage were 41.77, 42.78, 46.12 and 49.78% in each group (p<0.05), also fat percentage were a significant increase according to feeding levels. In economic efficacy, T4 was higher than other treatment groups. In conclusion, both rice straw and concentrate feed ab libitum are good for feeding and management system on Korean black goats.
Success in molecular breeding for better adapted varieties to environmental stresses depend upon the concerted efforts by various research including tissue culture, transformation, genetics and breeding. In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators on callus induction and plant regeneration were investigated with mature seeds. The highest callus induction frequency was observed when the mature seeds were cultured on MS medium supplemented with 5 mg/L 2,4-D. The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.
This study was conducted to determine the effect of dietary whole-crop barley silage on the daily feed intake, daily weight gain, velvet antler yield and economic efficacy in elk during growth. A total of 21 elk (average BW 303 kg, 5 years old) were allotted randomly to three treatments. The three treatments were T1 (hay diet), T2 (whole-crop barley silage), and T3 (50% hay + 50% whole-crop barley silage). The average daily weight gain of T3 was higher than the others throughout the entire experimental period. The average daily feed intake of elk in decreasing order was as follows; T1>T3>T2. The velvet antler yield was higher in T3 group (8,585 g) compared with T1 (8,037 g) and T2 (7,713 g). However, there were no significant differences in the average daily feed intake, average daily weight gain, or velvet antler yield (p>0.05). In economic efficacy, T3 was gained about 29~43% more value than T1 or T2. In conclusion, the mixed feeding of 50% hay and 50% whole-crop barley silage was more effective than feeding hay of barley alone, in terms of average daily weight gain, velvet antler yield, and economic efficacy in elk.
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