In Vitro Plant Regeneration of Siberian Wildrye Grass from Mature Seed-derived Callus

Abstract

Success in molecular breeding for better adapted varieties to environmental stresses depend upon the concerted efforts by various research including tissue culture, transformation, genetics and breeding. In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators on callus induction and plant regeneration were investigated with mature seeds. The highest callus induction frequency was observed when the mature seeds were cultured on MS medium supplemented with 5 mg/L 2,4-D. The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.

Optimization of tall fescue (Festuca arundinacea) callus induction and regeneration in culture medium containing ZnO and Ag nanoparticles

“…In this experiment, different levels of 2.4-D were used as auxin, and the level of 1 mg l − 1 showed the highest percentage of callus formation and the speed of callus formation. In one experiment, 5 mg l − 1 of 2.4-D was the ideal concentration for inducing callus from mature Siberian wild grass seeds (Lee et al, 2012). Callus development and regeneration capacity are enhanced when auxin is combined with low concentrations of cytokinins, such as 6-benzyl amino purine (BAP) (Gaba., 2005).…”