Summary
The corn pathogen Ustilago maydis requires its host plant maize for development
and completion of its sexual cycle. We have identified the fungal mig2–1
gene as being specifically expressed during this biotrophic stage. Intriguingly,
mig2–1 is part of a gene cluster comprising five highly homologous
and similarly regulated genes designated mig2–1 to mig2–5.
Deletion analysis of the mig2–1 promoter provides evidence for negative
and positive regulation. The predicted polypeptides of all five genes lack significant
homologies to known genes but have characteristic N‐terminal secretion sequences.
The secretion signals of mig2–1 and mig2–5 were shown
to be functional, and secretion of a full length Mig2–1‐eGFP fusion protein
to the extracellular space was demonstrated. The central domains of the Mig2 proteins
are highly variable whereas the C‐termini are strongly conserved and share a characteristic
pattern of eight cysteine residues. The mig2 gene cluster was conserved in
a wide collection of U. maydis strains. Interestingly, some U. maydis
isolates from South America had lost the mig2–4 gene as a result of
a homologous recombination event. Furthermore, the related Ustilago scitaminea
strain, which is pathogenic on sugar cane, appears to lack the mig2 cluster.
We describe a model of how the mig2 cluster might have evolved and discuss its possible role in governing host interaction.
The valorization of integrated steelworks process off-gases as feedstock for synthesizing methane and methanol is in line with European Green Deal challenges. However, this target can be generally achieved only through process off-gases enrichment with hydrogen and use of cutting-edge syntheses reactors coupled to advanced control systems. These aspects are addressed in the RFCS project i3upgrade and the central role of hydrogen was evident from the first stages of the project. First stationary scenario analyses showed that the required hydrogen amount is significant and existing renewable hydrogen production technologies are not ready to satisfy the demand in an economic perspective. The poor availability of low-cost green hydrogen as one of the main barriers for producing methane and methanol from process off-gases is further highlighted in the application of an ad-hoc developed dispatch controller for managing hydrogen intensified syntheses in integrated steelworks. The dispatch controller considers both economic and environmental impacts in the cost function and, although significant environmental benefits are obtainable by exploiting process off-gases in the syntheses, the current hydrogen costs highly affect the dispatch controller decisions. This underlines the need for big scale green hydrogen production processes and dedicated green markets for hydrogen-intensive industries, which would ensure easy access to this fundamental gas paving the way for a C-lean and more sustainable steel production.
To achieve the greenhouse gas reduction targets formulated in the European Green Deal, energy- and resource-intensive industries such as the steel industry will have to adapt or convert their production. In the long term, new technologies are promising. However, carbon capture storage and utilization solutions could be considered as short-term retrofitting solutions for existing steelworks. In this context, this paper presents a first experimental demonstration of an approach to the utilization of process off-gases generated in a steelworks by producing methane and methanol in hydrogen-intensified syntheses. Specifically, the integration of two methane synthesis reactors and one methanol synthesis reactor into a steel plant is experimentally simulated. An innovative monitoring and control tool, namely, a dispatch controller, simulates the process off-gas production using a digital twin of the steel plant and optimizes its distribution to existing and new consumers. The operating states/modes of the three reactors resulting from the optimization problem to be solved by the dispatch controller are distributed in real time via an online OPC UA connection to the corresponding experimental plants or their operators and applied there in a decentralized manner. The live coupling test showed that operating values for the different systems can be distributed in parallel from the dispatch controller to the test rigs via the established communication structure without loss. The calculation of a suitable control strategy is performed with a time resolution of one minute, taking into account the three reactors and the relevant steelworks components. Two of each of the methane/methanol synthesis reactors were operated error-free at one time for 10 and 7 h, respectively, with datasets provided by the dispatch controller. All three reactor systems were able to react quickly and stably to dynamic changes in the load or feed gas composition. Consistently high conversions and yields were achieved with low by-product formation.
This work investigates the cost-efficient integration of renewable hydrogen into steelworks for the production of methane and methanol as an efficient way to decarbonize the steel industry. Three case studies that utilize a mixture of steelworks off-gases (blast furnace gas, coke oven gas, and basic oxygen furnace gas), which differ on the amount of used off-gases as well as on the end product (methane and/or methanol), are analyzed and evaluated in terms of their economic performance. The most influential cost factors are identified and sensitivity analyses are conducted for different operating and economic parameters. Renewable hydrogen produced by PEM electrolysis is the most expensive component in this scheme and responsible for over 80% of the total costs. Progress in the hydrogen economy (lower electrolyzer capital costs, improved electrolyzer efficiency, and lower electricity prices) is necessary to establish this technology in the future.
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